Document Type
Article
Publication Date
5-3-2022
Abstract
Vaccines have generally been developed with limited insight into their molecular impact. While systems vaccinology enables characterization of mechanisms of action, these tools have yet to be applied to infants, who are at high risk of infection and receive the most vaccines. Bacille Calmette-Guérin (BCG) protects infants against disseminated tuberculosis (TB) and TB-unrelated infections via incompletely understood mechanisms. We employ mass-spectrometry-based metabolomics of blood plasma to profile BCG-induced infant responses in Guinea-Bissau in vivo and the US in vitro. BCG-induced lysophosphatidylcholines (LPCs) correlate with both TLR-agonist- and purified protein derivative (PPD, mycobacterial antigen)-induced blood cytokine production in vitro, raising the possibility that LPCs contribute to BCG immunogenicity. Analysis of an independent newborn cohort from The Gambia demonstrates shared vaccine-induced metabolites, such as phospholipids and sphingolipids. BCG-induced changes to the plasma lipidome and LPCs may contribute to its immunogenicity and inform the development of early life vaccines.
Recommended Citation
Diray-Arce, Joann; Angelidou, Asimenia; Jensen, Kristoffer Jarlov; Conti, Maria Giulia; Kelly, Rachel S; Pettengill, Matthew; Liu, Mark; van Haren, Simon D; McCulloch, Scott D; Michelloti, Greg; Idoko, Olubukola; Kollmann, Tobias R; Kampmann, Beate; Steen, Hanno; Ozonoff, Al; Lasky-Su, Jessica; Benn, Christine S; and Levy, Ofer, "Bacille Calmette-Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro" (2022). Department of Pathology, Anatomy, and Cell Biology Faculty Papers. Paper 359.
https://jdc.jefferson.edu/pacbfp/359
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
PubMed ID
35508141
Language
English
Comments
This article is the author’s final published version in Cell reports, Volume 39, Issue 5, May 2022, Pages 110772.
The published version is available at https://doi.org/10.1016/j.celrep.2022.110772. Copyright © Diray-Arce et al.