Document Type
Article
Publication Date
7-23-2021
Abstract
We developed and analyzed a single cell scale anatomical map of the rat intrinsic cardiac nervous system (ICNS) across four male and three female hearts. We find the ICNS has a reliable structural organizational plan across individuals that provide the foundation for further analyses of the ICNS in cardiac function and disease. The distribution of the ICNS was evaluated by 3D visualization and data-driven clustering. The pattern, distribution, and clustering of ICNS neurons across all male and female rat hearts is highly conserved, demonstrating a coherent organizational plan where distinct clusters of neurons are consistently localized. Female hearts had fewer neurons, lower packing density, and slightly reduced distribution, but with identical localization. We registered the anatomical data from each heart to a geometric scaffold, normalizing their 3D coordinates for standardization of common anatomical planes and providing a path where multiple experimental results and data types can be integrated and compared.
Recommended Citation
Leung, Clara; Robbins, Shaina; Moss, Alison; Heal, Maci; Osanlouy, Mahyar; Christie, Richard; Farahani, Navid; Monteith, Corey; Chen, Jin; Hunter, Peter; Tappan, Susan; Vadigepalli, Rajanikanth; Cheng, Zixi Jack; and Schwaber, James S., "3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system." (2021). Department of Pathology, Anatomy, and Cell Biology Faculty Papers. Paper 322.
https://jdc.jefferson.edu/pacbfp/322
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
PubMed ID
34355144
Language
English
Comments
This article is the authors’ final published version in iScience, Volume 24, Issue 7, July 2021, Article number 102795.
The published version is available at https://doi.org/10.1016/j.isci.2021.102795. Copyright © Leung et al.