Loss of Expression of SWI/SNF Chromatin Remodeling Complex Components and SETD2 are Heterogeneous, Widespread, and Co-Occur in Clear Cell Renal Cell Carcinoma

Authors

Wei Jiang, MD, PhD, Thomas Jefferson UniversityFollow
Essel Dulaimi, Fox Chase Cancer Center/Temple UniversityFollow
Theodore Parsons, Department of Pathology, Anatomy, and Cell Biology, Jefferson Medical College of Thomas Jefferson University, Philadelphia, PAFollow
Qiong Wang, Thomas Jefferson UniversityFollow
Karthik Devarajan, Fox Chase Cancer Center/Temple UniversityFollow
Raymond O'Neill, Thomas Jefferson UniversityFollow
Charalambos C. Solomides, MD, Thomas Jefferson University HospitalFollow
Stephen C Peiper, PhD, Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, United States of AmericaFollow
Jospeh R. Testa, Fox Chase Cancer Center/Temple UniversityFollow
Robert Uzzo, Fox Chase Cancer Center/Temple UniversityFollow
Haifeng Yang, Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, United States of AmericaFollow

Document Type

Poster

Publication Date

4-20-2015

Abstract

Background

The subunits of the SWI/SNF chromatin remodeling complex have one of the highest mutation rate in human cancers (see Figure 1). Polybromo-1 (PBRM1), a subunit of the SWI/SNF complex, is the second most mutated gene (40%) in clear cell Renal Cell Carcinoma (ccRCC). ARID1A, another subunit that competes with PBRM1 for binding to the complex, was infrequently mutated. The protein expressions of BRG1 and BRM (two catalytic subunits of the complex), and SETD2, a histone modifier (10-15% mutation rate), are not known in ccRCC. In this study we examined their protein expressions by immunohistochemistry (IHC) in tissue microarrays (TMAs), and investigated the prevalence of loss of expression, heterogeneity, and the potential co-loss patterns of these proteins.

Recommended Citation

Jiang, MD, PhD, Wei; Dulaimi, Essel; Parsons, Theodore; Wang, Qiong; Devarajan, Karthik; O'Neill, Raymond; Solomides, MD, Charalambos C.; Peiper, PhD, Stephen C; Testa, Jospeh R.; Uzzo, Robert; and Yang, Haifeng, "Loss of Expression of SWI/SNF Chromatin Remodeling Complex Components and SETD2 are Heterogeneous, Widespread, and Co-Occur in Clear Cell Renal Cell Carcinoma" (2015). Department of Pathology, Anatomy, and Cell Biology Faculty Papers. Paper 161.
https://jdc.jefferson.edu/pacbfp/161