Document Type

Article

Publication Date

5-11-2023

Comments

This article is the author's final published version in Cell Reports Methods, Volume 3, Issue 5, 22 May 2023, Article number 100477.

The published version is available at https://doi.org/10.1016/j.crmeth.2023.100477. Copyright © 2023 The Author(s).

Abstract

Chemical neurotransmission occurs at specialized contacts where neurotransmitter release machinery apposes neurotransmitter receptors to underlie circuit function. A series of complex events underlies preand postsynaptic protein recruitment to neuronal connections. To better study synaptic development in individual neurons, we need cell-type-specific strategies to visualize endogenous synaptic proteins. Although presynaptic strategies exist, postsynaptic proteins remain less studied because of a paucity of cell-type-specific reagents. To study excitatory postsynapses with cell-type specificity, we engineered dlg1[4K], a conditionally labeled marker of Drosophila excitatory postsynaptic densities. With binary expression systems, dlg1[4K] labels central and peripheral postsynapses in larvae and adults. Using dlg1[4K], we find that distinct rules govern postsynaptic organization in adult neurons, multiple binary expression systems can concurrently label pre- and postsynapse in a cell-type-specific manner, and neuronal DLG1 can sometimes localize presynaptically. These results validate our strategy for conditional postsynaptic labeling and demonstrate principles of synaptic organization.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Additional Files
1-s2.0-S2667237523001005-mmc1.pdf (7657 kB)
Supplementary Materials
1-s2.0-S2667237523001005-mmc2.pdf (18040 kB)
Full Article with supplements

Language

English

Download

Share

COinS