Concomitant Retrograde Coronary Venous Infusion of Basic Fibroblast Growth Factor Enhances Engraftment and Differentiation of Bone Marrow Mesenchymal Stem Cells for Cardiac Repair after Myocardial Infarction.

Authors

• Xiao Wang, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases
• Lei Zhen, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases
• Huangtai Miao, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases
• Qiwei Sun, Department of Ultrasound, Beijing Anzhen Hospital, Capital Medical University
• Ya Yang, Department of Ultrasound, Beijing Anzhen Hospital, Capital Medical University
• Bin Que, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases
• Edmundo Patricio Lopes Lao, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases
• Xingxin Wu, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases
• Hongmei Ren, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases
• Shutian Shi, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases
• Wayne Bond Lau, Department of Emergency Medicine, Thomas Jefferson University, 1015 Walnut Street, Philadelphia, PA, 19107, USAFollow
• Xin-Liang Ma, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases; Department of Emergency Medicine, Thomas Jefferson UniversityFollow
• Changsheng Ma, Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University
• Shaoping Nie, Emergency and Critical Care Center, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung, and Blood Vessel Diseases

Document Type

Article

Publication Date

6-8-2015

Comments

This article has been peer reviewed. It was published in: Theranostics.

Volume 5, Issue 9, 2015, Pages 995-1006.

The published version is available at DOI: 10.7150/thno.11607

Copyright © 2015 Ivyspring International Publisher

Abstract

AIM: Basic fibroblast growth factor (bFGF) increases the migration and viability of bone marrow mesenchymal stem cells (MSCs) in vitro. Retrograde coronary venous infusion can provide both increased regional bFGF concentrations and homogeneous cell dissemination. We determined whether retrograde delivery of bFGF enhances the potency of transplanted MSCs for cardiac repair in a canine infarct model.

METHODS AND RESULTS: Under hypoxic conditions, cellular migration was significantly increased in MSCs co-cultured with bFGF compared to vascular endothelial growth factor or insulin-like growth factor, and bFGF promoted MSCs differentiation into a cardiomyocyte phenotype. A canine infarct model was employed by coronary ligation. One week later, animals were subjected to retrograde infusion of combination bFGF (200ng/mL) and MSCs (1×10(8) cells) (n=5), MSCs (1×10(8) cells, n=5), bFGF (200ng/mL, n=5), or placebo (phosphate-buffered saline, n=3). Four weeks after infusion, only the bFGF+MSCs therapy exhibited significantly increased left ventricular ejection fraction (LVEF) by echocardiography (p

CONCLUSIONS: Retrograde coronary venous bFGF infusion augments engraftment and differentiation capacity of transplanted MSCs, recovering cardiac function and preventing adverse remodeling. This novel combined treatment and delivery method is a promising strategy for cardiac repair after ischemic injury.

Recommended Citation

Wang, Xiao; Zhen, Lei; Miao, Huangtai; Sun, Qiwei; Yang, Ya; Que, Bin; Lopes Lao, Edmundo Patricio; Wu, Xingxin; Ren, Hongmei; Shi, Shutian; Lau, Wayne Bond; Ma, Xin-Liang; Ma, Changsheng; and Nie, Shaoping, "Concomitant Retrograde Coronary Venous Infusion of Basic Fibroblast Growth Factor Enhances Engraftment and Differentiation of Bone Marrow Mesenchymal Stem Cells for Cardiac Repair after Myocardial Infarction." (2015). Department of Emergency Medicine Faculty Papers. Paper 40.
https://jdc.jefferson.edu/emfp/40

PubMed ID

26155315