Document Type
Article
Publication Date
8-22-2018
Abstract
Neuronal cell morphogenesis depends on proper regulation of microtubule-based transport, but the underlying mechanisms are not well understood. Here, we report our study of MAP7, a unique microtubule-associated protein that interacts with both microtubules and the motor protein kinesin-1. Structure-function analysis in rat embryonic sensory neurons shows that the kinesin-1 interacting domain in MAP7 is required for axon and branch growth but not for branch formation. Also, two unique microtubule binding sites are found in MAP7 that have distinct dissociation kinetics and are both required for branch formation. Furthermore, MAP7 recruits kinesin-1 dynamically to microtubules, leading to alterations in organelle transport behaviors, particularly pause/speed switching. As MAP7 is localized to branch sites, our results suggest a novel mechanism mediated by the dual interactions of MAP7 with microtubules and kinesin-1 in the precise control of microtubule-based transport during axon morphogenesis.
Recommended Citation
Tymanskyj, Stephen R.; Yang, Benjamin; Verhey, Kristen J.; and Ma, Le, "MAP7 regulates axon morphogenesis by recruiting kinesin-1 to microtubules and modulating organelle transport." (2018). Department of Neuroscience Faculty Papers. Paper 36.
https://jdc.jefferson.edu/department_neuroscience/36
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Language
English
Comments
This article has been peer reviewed. It is the author’s final published version in eLife, Volume 7, August 2018, Article number e36374.
The published version is available at https://doi.org/10.7554/eLife.36374. Copyright © Tymanskyj et al.