Document Type
Article
Publication Date
Winter 2-25-2009
Abstract
Polypeptide variants of the HA1 antigenic domain of the H5N1 avian influenza virus hemagglutinin (HA) molecule were produced in plants using transient and stable expression systems and fused with His/c-myc tags or with mouse or human Fc antibody fragments. The resulting peptides were purified and used for intramuscular immunization of mice. While the recombinant HA1 variants induced a significant serum humoral immune response in the mice, none of the HA1 preparations induced virus-neutralizing antibodies. Fusion with the Fc fragment improved overall yield of the constructs and allowed purification requiring only a single step, but led to no detectable fusion-related enhancement of immunogenicity or quality of immune response.
Recommended Citation
Spitsin, S; Andrianov, V; Pogrebnyak, N; Smirnov, Y; Borisjuk, N; Portocarrero, C; Veguilla, V; Koprowski, H; and Golovkin, M, "Immunological assessment of plant-derived avian flu H5/HA1 variants." (2009). Department of Cancer Biology Faculty Papers. Paper 18.
https://jdc.jefferson.edu/cbfp/18
Figure_1_Flu_corrected.pdf
Figure_2_flu.pdf (197 kB)
Figure_2_flu.pdf
PubMed ID
19162113
Comments
This article has been peer reviewed. It is the authors' final version prior to publication in Vaccine. Volume 27, Issue 9, February 2009, Pages 1289-1292. The published version is available at . DOI: 10.1016/j.vaccine.2008.12.050. Copyright © Elsevier Inc..