Document Type
Article
Publication Date
1-1-2018
Abstract
Smk1 is a meiosis-specific MAP kinase (MAPK) in budding yeast that is required for spore formation. It is localized to prospore membranes (PSMs), the structures that engulf haploid cells during meiosis II (MII). Similar to canonically activated MAPKs, Smk1 is controlled by phosphorylation of its activation-loop threonine (T) and tyrosine (Y). However, activation loop phosphorylation occurs via a noncanonical two-step mechanism in which 1) the cyclin-dependent kinase activating kinase Cak1 phosphorylaytes T207 during MI, and 2) Smk1 autophosphorylates Y209 as MII draws to a close. Autophosphorylation of Y209 and catalytic activity for substrates require Ssp2, a meiosis-specific protein that is translationally repressed until anaphase of MII. Ama1 is a meiosis-specific targeting subunit of the anaphase-promoting complex/cyclosome that regulates multiple steps in meiotic development, including exit from MII. Here, we show that Ama1 activates autophosphorylation of Smk1 on Y209 by promoting formation of the Ssp2/Smk1 complex at PSMs. These findings link meiotic exit to Smk1 activation and spore wall assembly.
Recommended Citation
Omerza, Gregory; Tio, Chong Wai; Philips, Timothy; Diamond, Aviva; Neiman, Aaron M.; and Winter, Edward, "The meiosis-specific Cdc20 family-member Ama1 promotes binding of the Ssp2 activator to the Smk1 MAP kinase." (2018). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 131.
https://jdc.jefferson.edu/bmpfp/131
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 License.
PubMed ID
29118076
Comments
This article has been peer reviewed. It is the author’s final published version in Molecular Biology of the Cell
Volume 29, Issue 1, January 2018, Pages 66-74.
The published version is available at DOI: 10.1091/mbc.E17-07-0473. Copyright © Omerza et al.