Document Type

Article

Publication Date

7-1-2025

Comments

This article is the author's final published version in RNA Biology, Volume 22, Issue 1, July, 2025, Pages 1-9.

The published version is available at https://doi.org/10.1080/15476286.2025.2525886.

Copyright © 2025 The Author(s).

Abstract

Monocytes, a type of leukocytes, are key players in immune responses, transitioning to macrophages at infection sites. Differentiation, which is crucial for macrophage survival and resistance to apoptosis, is a tightly regulated process. Based on our earlier findings implicating the 5'-fragment of tRNAHisGUG (5'-HisGUG) in macrophage biology, we specifically focused on these fragments to investigate its regulation during monocyte-to-macrophage differentiation and its role in macrophage survival. We performed small RNA sequencing during monocyte-to-macrophage differentiation using THP-1 cells and identified 5'-HisGUG as a prominently regulated tDR. Notably, transfection of 5'-HisGUG in macrophages decreased survival under apoptotic stress. We propose that 5'-HisGUG can also act as an obstructive RNA (oRNA), and its reduction is critical for macrophage survival under stress. © 2025 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

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PubMed ID

40575931

Language

English

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