Document Type
Article
Publication Date
12-10-2024
Abstract
Angiogenin (Ang), an endoribonuclease belonging to the RNase A superfamily, cleaves the anticodon-loops of tRNAs to produce tRNA-half molecules. Although previous studies have demonstrated the involvement of Ang in the pathobiology of neurodegenerative disorders, the characterization of Ang-generated tRNA halves in neuronal cells remains limited. This is partly due to the technical limitations of standard RNA-seq methods, which cannot capture Ang-generated RNAs containing a 2',3'-cyclic phosphate (cP). In this report, we established an Ang-treatment model using SH-SY5Y, a human neuroblastoma cell line, and demonstrated Ang-dependent accumulation of tRNA halves. By performing cP-RNA-seq, which selectively captures cP-containing RNAs, we identified Ang-generated tRNA halves and the specific cleavage positions within tRNA anticodon-loops responsible for their generation. Our results provide insights into the anticodon-loop cleavage and the selective production of a specific subset of tRNA halves by Ang.
Recommended Citation
Shigematsu, Megumi; Matsubara, Ryuma; Gumas, Justin; Kawamura, Takuya; and Kirino, Yohei, "Angiogenin-Catalyzed Cleavage Within tRNA Anticodon-Loops Identified by cP-RNA-seq" (2024). Computational Medicine Center Faculty Papers. Paper 60.
https://jdc.jefferson.edu/tjucompmedctrfp/60
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
PubMed ID
39658364
Language
English
Comments
This article is the author's final published version in Bioscience, Biotechnology and Biochemistry, Volume 89, Issue 3, March 2025, pages 398-405.
The published version is available at https://doi.org/10.1093/bbb/zbae192.
Copyright © The Author(s) 2024.