Document Type
Article
Publication Date
8-28-2020
Abstract
Mitochondria generate most cellular energy via oxidative phosphorylation. Twenty-two species of mitochondrial (mt-)tRNAs encoded in mtDNA translate essential subunits of the respiratory chain complexes. mt-tRNAs contain post-transcriptional modifications introduced by nuclear-encoded tRNA-modifying enzymes. They are required for deciphering genetic code accurately, as well as stabilizing tRNA. Loss of tRNA modifications frequently results in severe pathological consequences. Here, we perform a comprehensive analysis of post-transcriptional modifications of all human mt-tRNAs, including 14 previously-uncharacterized species. In total, we find 18 kinds of RNA modifications at 137 positions (8.7% in 1575 nucleobases) in 22 species of human mt-tRNAs. An up-to-date list of 34 genes responsible for mt-tRNA modifications are provided. We identify two genes required for queuosine (Q) formation in mt-tRNAs. Our results provide insight into the molecular mechanisms underlying the decoding system and could help to elucidate the molecular pathogenesis of human mitochondrial diseases caused by aberrant tRNA modifications.
Recommended Citation
Suzuki, Takeo; Yashiro, Yuka; Kikuchi, Ittoku; Ishigami, Yuma; Saito, Hironori; Matsuzawa, Ikuya; Okada, Shunpei; Mito, Mari; Iwasaki, Shintaro; Ma, Ding; Zhao, Xuewei; Asano, Kana; Lin, Huan; Kirino, Yohei; Sakaguchi, Yuriko; and Suzuki, Tsutomu, "Complete chemical structures of human mitochondrial tRNAs" (2020). Computational Medicine Center Faculty Papers. Paper 27.
https://jdc.jefferson.edu/tjucompmedctrfp/27
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
PubMed ID
32859890
Language
English
Comments
This article is the author’s final published version in Nature Communications, Volume 11, Issue 1, August 2020, Article number 4269
The published version is available at https://doi.org/10.1038/s41467-020-18068-6. Copyright © Suzuki et al.