Evaluating the Sensitivity of the NanoStrong nCounter® Analysis System to Determine Gene Expression Changes Associated with Chemotherapy Treatment

Authors

Katerina Dukleska, MD, Thomas Jefferson UniversityFollow
Christopher W. Schultz, BS, Thomas Jefferson UniversityFollow
Mahsa Zarei, PhD, Thomas Jefferson UniversityFollow
David McKeown, MD, Thomas Jefferson UniversityFollow
Charles J. Yeo, MD, Thomas Jefferson UniversityFollow
Jonathan Brody, PhD, Thomas Jefferson UniversityFollow
Jordan M. Winter, MD, Thomas Jefferson UniversityFollow

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Description

Introduction

• Pancreatic ductal adenocarcinoma (PDA) remains a deadly disease with a 5-year survival of 8% for all stages combined (Siegel RL et al.). Currently, it’s the third leading cause of cancer-related deaths in the United States and by 2020 it is projected to become the second leading cause (Rahib L, et al).
• The poor prognosis in PDA is in part due to the limited therapies that are currently available.
• This highlights the importance of high-throughput technologies for gene expression analyses and drug screens.
• A recently developed NanoString Technologies nCounter® analysis system, an example of high-throughput technology, utilizes a color-coded barcode to directly measure multiple mRNA transcripts simultaneously.
• Our ability to study RNA expression has classically been limited by low detection rate (microarrays) or high cost (RNA-Seq).
• We utilized the nCounter® analysis system to measure changes in cancer-associated gene expression in PDA cell lines caused by different drug treatment.
• We then validated these results using traditional assays to evaluate the sensitivity of the nCounter® analysis system.

Poster presented at: Metro Philadelphia Chapter of the American College of Surgeons in Philadelphia PA, United States.

Publication Date

5-22-2000

Keywords

Evaluating the Sensitivity of the NanoStrong nCounter® Analysis System to Determine Gene Expression Changes Associated with Chemotherapy Treatment, poster, Department of Surgery, Thomas Jefferson University

Disciplines

Surgery

Recommended Citation

Dukleska, MD, Katerina; Schultz, BS, Christopher W.; Zarei, PhD, Mahsa; McKeown, MD, David; Yeo, MD, Charles J.; Brody, PhD, Jonathan; and Winter, MD, Jordan M., "Evaluating the Sensitivity of the NanoStrong nCounter® Analysis System to Determine Gene Expression Changes Associated with Chemotherapy Treatment" (2000). Department of Surgery Posters. 9.
https://jdc.jefferson.edu/surgeryposters/9