Document Type
Presentation
Publication Date
8-8-2013
Abstract
The mechanism by which the hapten 2,4-dinitrophenyl (DNP) mediates cell death is unclear according to current literature. To determine how DNP mediates death in the B16F10 murine melanoma cell line in vitro, cells were modified with 2,4-dinitro-1-fluorobenzene (DNFB). Following modification, cell lysates were collected and analyzed via Western blottings with known apoptotic or necrotic markers. Our results indicated that DNFB treated cells do not express the apoptotic marker Cleaved Caspase-3 and have no change in levels of the necrotic marker Cyclophilin A. We conclude that DNFB is not inducing apoptosis in B16F10 cells. Future research will further examine the possibility of necrotic cell death using a biomarker other then Cyclophilin A.
Recommended Citation
Thelmo, Franklin, "Unraveling the Mechanism of 2,4-Dinitrofluorobenzene-Associated Cell Death" (2013). Summer Training Program in Cancer Immunotherapy. Presentation 9.https://jdc.jefferson.edu/summercancerimmunotherapy/9
Final Paper
Comments
Paper is attached at bottom of page.