Document Type


Publication Date

August 2007


This article has been peer reviewed. It was published in the Journal of Bacteriology 189(16):6074-6079, August 2007. The published version is available at Copyright 2007 by the American Society for Microbiology, posted here by permission.


A novel and functional conjugative transfer system identified in O119:H2 enteropathogenic Escherichia coli (EPEC) strain MB80 by subtractive hybridization is encoded on a large multidrug resistance plasmid, distinct from the well-described EPEC adherence factor (EAF) plasmid. Variants of the MB80 conjugative resistance plasmid were identified in other EPEC strains, including the prototypical O111:NM strain B171, from which the EAF plasmid has been sequenced. This separate large plasmid and the selective advantage that it confers in the antibiotic era have been overlooked because it comigrates with the virulence plasmid on conventional gels.

MB80_restplasmid_v16_supptables_revised_4.doc (110 kB)
Table S2, BLAST hits for MB80 plasmid sample sequence runs; Table S3, primer table