Assessment of early tumor response to cytotoxic chemotherapy with dynamic contrast-enhanced ultrasound in human breast cancer xenografts.

Authors

Jian-Wei Wang, State Key Laboratory of Oncology in South China, Department of Ultrasound, Sun Yat-Sen University Cancer Center
Wei Zheng, State Key Laboratory of Oncology in South China, Department of Ultrasound, Sun Yat-Sen University Cancer Center
Ji-Bin Liu, Department of Radiology, Thomas Jefferson UniversityFollow
Yao Chen, State Key Laboratory of Oncology in South China, Department of Ultrasound, Sun Yat-Sen University Cancer Center
Long-Hui Cao, State Key Laboratory of Oncology in South China, Department of Anesthesiology, Sun Yat-Sen University Cancer CenterFollow
Rong-Zhen Luo, State Key Laboratory of Oncology in South China, Department of Pathology, Sun Yat-Sen University Cancer Center
An-Hua Li, State Key Laboratory of Oncology in South China, Department of Ultrasound, Sun Yat-Sen University Cancer Center
Jian-Hua Zhou, State Key Laboratory of Oncology in South China, Department of Ultrasound, Sun Yat-Sen University Cancer Center

Document Type

Article

Publication Date

3-1-2013

Comments

This article has been peer reviewed and is published in PLoS One.

Volume 8, Issue 3, 1 March 2013, Article numbere58274.

The published version is available at DOI: 10.1371/journal.pone.0058274. © 2013 Wang et al.

Abstract

There is a strong need to assess early tumor response to chemotherapy in order to avoid adverse effects from unnecessary chemotherapy and allow early transition to second-line therapy. This study was to quantify tumor perfusion changes with dynamic contrast-enhanced ultrasound (CEUS) in the evaluation of early tumor response to cytotoxic chemotherapy. Sixty nude mice bearing with MCF-7 breast cancer were administrated with either adriamycin or sterile saline. CEUS was performed on days 0, 2, 4 and 6 of the treatment, in which time-signal intensity (SI) curves were obtained from the intratumoral and depth-matched liver parenchyma. Four perfusion parameters including peak enhancement (PE), area under the curve of wash-in (WiAUC), wash-in rate (WiR) and wash-in perfusion index (WiPI) were calculated from perfusion curves and normalized with respect to perfusion of adjacent liver parenchyma. Histopathological analysis was conducted to evaluate tumor perfusion, tumor cell density, microvascular density (MVD) and proliferating cell density. Significant decreases of tumor normalized perfusion parameters (i.e., nPE, nWiAUC, nWiR and nWiPI) were noticed between adriamycin-treated and control groups (P0.05). Significant decreases of tumor perfusion, tumor cell density, MVD and proliferating cell density were seen in adrianycin-treated group 2 days after therapy when compared to control group (P

Recommended Citation

Wang, Jian-Wei; Zheng, Wei; Liu, Ji-Bin; Chen, Yao; Cao, Long-Hui; Luo, Rong-Zhen; Li, An-Hua; and Zhou, Jian-Hua, "Assessment of early tumor response to cytotoxic chemotherapy with dynamic contrast-enhanced ultrasound in human breast cancer xenografts." (2013). Department of Radiology Faculty Papers. Paper 26.
https://jdc.jefferson.edu/radiologyfp/26

PubMed ID

23469274