Document Type
Article
Publication Date
8-1-2026
Abstract
The concept of the "biomolecular corona" (BC) was first introduced to describe the spontaneous absorption of molecules, mainly proteins, onto the surface of synthetic nanoparticles upon exposure to biological fluids. More recently, the same term has been used to describe biomolecules that dynamically interact with the surface of extracellular vesicles (EVs) during their biogenesis, secretion, or upon exposure to intracellular or extracellular milieus, including biological fluids. The EV biomolecular corona is a key determinant of EV identity, shaping their cellular uptake, biodistribution, and functional outcomes in both physiological and pathological contexts. EVs, in contrast to synthetic nanoparticles, introduce distinct challenges and perspectives due to their biological origin, innate heterogeneity, and complex combination of physicochemical and biological properties. To address this evolving topic, the International Society for Extracellular Vesicles (ISEV) organized a dedicated workshop in Brescia, Italy, on 27th to 28th February, 2025. The event gathered selected participants from academia and industry, with expertise spanning synthetic nanomaterials, EVs, biophysics, and translational nanomedicine. The program included keynote lectures, oral presentations, posters, and a series of interactive roundtables structured around fundamentals of the biomolecular corona, EV corona biogenesis and composition, analytical and separation tools and clinical and diagnostic potential applications. This report summarizes the key scientific insights, methodological challenges, conceptual debates, and future perspectives that emerged from the workshop.
Recommended Citation
Paolini, Lucia; Tassoni, Selene; Al-Jamal, Khuloud T.; Banquy, Xavier; Beke-Somfai, Tamás; Bergese, Paolo; Boros, Fanni Annamária; Bottini, Massimo; Boyd, Ben; Busatto, Sara; Bussolati, Benedetta; Canepa, Ester; Castagnola, Valentina; Clayton, Aled; Dawson, Kenneth; El-Andaloussi, Samir; Försönits, András; Fortunato, Orazio; Frigerio, Roberto; Gabrielli, Martina; Grange, Cristina; Guescini, Michele; Hayashi, Yuya; Hemmingsen, Jonas Klejs; Kim, Seungmin; Kinga, Ilyes; Kyykallio, Heikki; Lenassi, Metka; Lima, Luize Goncalves; Mahoney, Mỹ G.; Vandenbroucke, Camille Menaceur; Möller, Andreas; Musicò, Angelo; Nelissen, Inge; Nogueira, Lucas Fabrício Bahia; Nöhammer, Christa; Parisse, Pietro; Ramassamy, Charles; Roballo, Kelly Cristine Santos; Salvati, Anna; Sancho-Albero, María; Shelke, Ganesh; Shirmast, Paniz; Sonallya, Tasvilla; Szigyártó, Imola Csilla; van Eijndhoven, Monique A. J.; Viitala, Tapani; Wang, Shan; Yan, Yan; Zendrini, Andrea; Buzás, Edit; and Radeghieri, Annalisa, "Biomolecular Corona of Extracellular Vesicles: Report on Isev Workshop" (2026). Department of Pharmacology, Physiology, and Cancer Biology Faculty Papers. Paper 72.
https://jdc.jefferson.edu/ppcbfp/72
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
Language
English
Comments
This article is the author's final published version in Journal of Extracellular Biology, Volume 5, Issue 8, 2026, Article Number e70172.
The published version is available at https://doi.org/10.1002/jex2.70172. Copyright © 2026 The Author(s).