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To optimize the pre-embedding IG in stable cell lines and primary adult cardiomyocytes to get the most effective visualization of endomembrane/associated proteins: endo/sarcoplasmic reticulum Ca2+ release channels IP3 receptors and ryanodine receptors (IP3R, and RyR2) and the mitochondrial fission protein Drp1.
pre-embedding, immunogold, transmission electron microscopy
Medical Immunology | Medicine and Health Sciences | Pathology
Nichtova, Zuzana; Fernandez-Sanz, Celia; Bartok, A.; De La Fuente, Sergio; Hajnóczky, György; Sheu, Shey-Shing; and Csordás, György, "Endomembrane-Associated Pre-embedding Immunogold Labeling" (2018). Department of Pathology, Anatomy, and Cell Biology Posters. 14.