Endomembrane-Associated Pre-embedding Immunogold Labeling

Authors

Zuzana Nichtova, Thomas Jefferson UniversityFollow
Celia Fernandez-Sanz, Thomas Jefferson UniversityFollow
A. Bartok, Thomas Jefferson University
Sergio De La Fuente, Thomas Jefferson UniversityFollow
György Hajnóczky, Thomas Jefferson UniversityFollow
Shey-Shing Sheu, Thomas Jefferson UniversityFollow
György Csordás, Thomas Jefferson UniversityFollow

Files

Description

Aim

To optimize the pre-embedding IG in stable cell lines and primary adult cardiomyocytes to get the most effective visualization of endomembrane/associated proteins: endo/sarcoplasmic reticulum Ca2+ release channels IP3 receptors and ryanodine receptors (IP3R, and RyR2) and the mitochondrial fission protein Drp1.

Publication Date

7-12-2018

Keywords

pre-embedding, immunogold, transmission electron microscopy

Disciplines

Medical Immunology | Medicine and Health Sciences | Pathology

Comments

Presented at the 2018 Princeton Nature Conference: Frontiers in Electron Microscopy

Recommended Citation

Nichtova, Zuzana; Fernandez-Sanz, Celia; Bartok, A.; De La Fuente, Sergio; Hajnóczky, György; Sheu, Shey-Shing; and Csordás, György, "Endomembrane-Associated Pre-embedding Immunogold Labeling" (2018). Department of Pathology, Anatomy, and Cell Biology Posters. 14.
https://jdc.jefferson.edu/pacbposters/14