Decorin-mediated inhibition of colorectal cancer growth and migration is associated with E-cadherin in vitro and in mice.

Authors

Xiuli Bi, Liaoning University; University of Illinois at Chicago
Nicole M Pohl, University of Illinois at Chicago
Zhibin Qian, Xinxiang Medical University
George R Yang, University of Illinois at Chicago
Yuan Gou, University of Illinois at Chicago
Grace Guzman, University of Illinois at Chicago
Andre Kajdacsy-Balla, University of Illinois at Chicago
Renato V Iozzo, Thomas Jefferson UniversityFollow
Wancai Yang, University of Illinois at Chicago; Xinxiang Medical University

Document Type

Article

Publication Date

2-1-2012

Comments

This is a peer reviewed article. The original version is published in Carcinogenisis, Volume 33, Issue 2, 2012, Pages 326-330. ©Oxford University Press

Abstract

Previous studies have shown that decorin expression is significantly reduced in colorectal cancer tissues and cancer cells, and genetic deletion of the decorin gene is sufficient to cause intestinal tumor formation in mice, resulting from a downregulation of p21, p27(kip1) and E-cadherin and an upregulation of β-catenin signaling [Bi,X. et al. (2008) Genetic deficiency of decorin causes intestinal tumor formation through disruption of intestinal cell maturation. Carcinogenesis, 29, 1435-1440]. However, the regulation of E-cadherin by decorin and its implication in cancer formation and metastasis is largely unknown. Using a decorin knockout mouse model (Dcn(-/-) mice) and manipulated expression of decorin in human colorectal cancer cells, we found that E-cadherin, a protein that regulates cell-cell adhesion, epithelial-mesenchymal transition and metastasis, was almost completely lost in Dcn(-/-) mouse intestine, and loss of decorin and E-cadherin accelerated colon cancer cell growth and invasion in Dcn(-/-) mice. However, increasing decorin expression in colorectal cancer cells attenuated cancer cell malignancy, including inhibition of cancer cell proliferation, promotion of apoptosis and importantly, attenuation of cancer cell migration. All these changes were linked to the regulation of E-cadherin by decorin. Moreover, overexpression of decorin upregulated E-cadherin through increasing of E-cadherin protein stability as E-cadherin messenger RNA and promoter activity were not affected. Co-immunoprecipitation assay showed a physical binding between decorin and E-cadherin proteins. Taken together, our results provide direct evidence that decorin-mediated inhibition of colorectal cancer growth and migration are through the interaction with and stabilization of E-cadherin.

Recommended Citation

Bi, Xiuli; Pohl, Nicole M; Qian, Zhibin; Yang, George R; Gou, Yuan; Guzman, Grace; Kajdacsy-Balla, Andre; Iozzo, Renato V; and Yang, Wancai, "Decorin-mediated inhibition of colorectal cancer growth and migration is associated with E-cadherin in vitro and in mice." (2012). Department of Pathology, Anatomy, and Cell Biology Faculty Papers. Paper 93.
https://jdc.jefferson.edu/pacbfp/93

PubMed ID

22159220