Perfluorochemical liquid-adenovirus suspensions enhance gene delivery to the distal lung.

Authors

Jeffrey A Kazzaz, Departments of Medicine and Pediatrics, SUNY Stony Brook School of Medicine, Winthrop University Hospital
Marlene S Strayer, Department of Pathology, Thomas Jefferson University, Medical CenterFollow
Jichuan Wu, Departments of Physiology and Pediatrics, Temple University, School of Medicine
Daniel J Malone, Departments of Physiology and Pediatrics, Temple University, School of Medicine
Hshi-Chi Koo, Departments of Physiology and Pediatrics, Temple University, School of Medicine
Thomas H Shaffer, Departments of Physiology and Pediatrics, Temple University, School of Medicine; Nemours Research Lung Center, Alfred I. DuPont Hospital for ChildrenFollow
Jonathan M Davis, Departments of Medicine and Pediatrics, SUNY Stony Brook School of Medicine, Winthrop University Hospital; Department of Pediatrics, Floating Hospital for Children, Tufts Medical Center
David S Strayer, Department of Pathology, Thomas Jefferson University, Medical CenterFollow
Marla R Wolfson, Departments of Physiology and Pediatrics, Temple University, School of Medicine; Center for Inflammation Translational and Clinical Lung Research (CILR), Temple University, School of Medicine

Document Type

Article

Publication Date

8-18-2011

Comments

This article has been peer reviewed. It was published in: Pulmonary Medicine.

2011, Article number 918036.

The published version is available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3159382/. DOI: 10.1155/2011/918036.

Copyright © 2011 Jeffrey A. Kazzaz et al.

Abstract

WE COMPARED LUNG DELIVERY METHODS OF RECOMBINANT ADENOVIRUS (RAD): (1) rAd suspended in saline, (2) rAd suspended in saline followed by a pulse-chase of a perfluorochemical (PFC) liquid mixture, and (3) a PFC-rAd suspension. Cell uptake, distribution, and temporal expression of rAd were examined using A549 cells, a murine model using luciferase bioluminescence, and histological analyses. Relative to saline, a 4X increase in transduction efficiency was observed in A549 cells exposed to PFC-rAd for 2-4 h. rAd transgene expression was improved in alveolar epithelial cells, and the level and distribution of luciferase expression when delivered in PFC-rAd suspensions consistently peaked at 24 h. These results demonstrate that PFC-rAd suspensions improve distribution and enhance rAd-mediated gene expression which has important implications in improving lung function by gene therapy.

Recommended Citation

Kazzaz, Jeffrey A; Strayer, Marlene S; Wu, Jichuan; Malone, Daniel J; Koo, Hshi-Chi; Shaffer, Thomas H; Davis, Jonathan M; Strayer, David S; and Wolfson, Marla R, "Perfluorochemical liquid-adenovirus suspensions enhance gene delivery to the distal lung." (2011). Department of Pathology, Anatomy, and Cell Biology Faculty Papers. Paper 138.
https://jdc.jefferson.edu/pacbfp/138

PubMed ID

21876799