Document Type
Article
Publication Date
6-1-2011
Abstract
Neutrophil recruitment via CXCR2 is required for innate and adaptive protective immunity to the larvae of Strongyloides stercoralis in mice. The goal of the present study was to determine the mechanism of CXCR2-mediated neutrophil recruitment to S. stercoralis. Mice deficient in the receptor for IL-17A and IL-17F, upstream mediators of CXCR2 ligand production, were infected with S. stercoralis larvae; there was no difference in larval survival, neutrophil recruitment, or production of CXCR2 ligands compared with wild type mice. In vivo and in vitro stimulation of neutrophils with S. stercoralis soluble extract resulted in significant neutrophil recruitment. In vitro assays demonstrated that the recruitment functioned through both chemokinesis and chemotaxis, was specific for CXCR2, and was a G protein-coupled response involving tyrosine kinase and PI3K. Finally, neutrophil stimulation with S. stercoralis soluble extract induced release of the CXCR2 ligands MIP-2 and KC from neutrophils, thereby potentially enhancing neutrophil recruitment.
Recommended Citation
O'Connell, Amy E.; Redding, Kevin; Hess, Jessica A.; Lok, James B.; Nolan, Thomas J.; and Abraham, David, "Soluble extract from the nematode Strongyloides stercoralis induces CXCR2 dependent/IL-17 independent neutrophil recruitment." (2011). Department of Microbiology and Immunology Faculty Papers. Paper 151.
https://jdc.jefferson.edu/mifp/151
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
PubMed ID
21315175
Language
English
Comments
This article is the authors' final version prior to publication in Microbes and Infection, Volume 13, Issue 6, June 2011, Pages 536-544.
The published version is available at https://doi.org/10.1016/j.micinf.2011.01.016. Copyright © O'Connell et al.