Protocol for Inducing Focal Striatal Stroke in Mice Using Photothrombosis

Authors

• Bridget R. Boyle, Thomas Jefferson UniversityFollow
• Eileen Collyer, Thomas Jefferson University
• Andrea P. Berghella, Thomas Jefferson UniversityFollow
• Elena Blanco-Suarez

Document Type

Article

Publication Date

4-7-2026

Comments

This article is the author’s final published version in STAR Protocols, Volume 7, Issue 2, 2026, Article number 104487.

The published version is available at https://doi.org/10.1016/j.xpro.2026.104487. Copyright © 2026 The Authors.

Abstract

Here, we present a protocol for inducing focal striatal stroke in mice using photothrombosis. We describe steps for photothrombotic striatal surgery, magnetic resonance imaging, open field behavioral assessment of stroke-induced motor deficits, and post-stroke monitoring. We then detail procedures for tissue collection and immunofluorescence. This protocol provides a standardized approach for consistently modeling striatal stroke in mice, enabling mechanistic studies and preclinical therapeutic testing.

Recommended Citation

Boyle, Bridget R.; Collyer, Eileen; Berghella, Andrea P.; and Blanco-Suarez, Elena, "Protocol for Inducing Focal Striatal Stroke in Mice Using Photothrombosis" (2026). Department of Neuroscience Faculty Papers. Paper 94.
https://jdc.jefferson.edu/department_neuroscience/94

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Language

English