Selective axonal transport through branch junctions is directed by growth cone signaling and mediated by KIF1/kinesin-3 motors.

Authors

Stephen R. Tymanskyj, Thomas Jefferson UniversityFollow
Bridget M. Curran, Thomas Jefferson UniversityFollow
Le Ma, Thomas Jefferson UniversityFollow

Document Type

Article

Publication Date

4-26-2022

Comments

This article is the author’s final published version in Cell reports, Volume 39, Issue 4, April 2022, Pages 110748.

The published version is available at https://doi.org/10.1016/j.celrep.2022.110748. Copyright © Tymanskyj et al.

Abstract

Development and function of nerve cells rely on the orchestration of microtubule-based transport from the cell body into distal axonal terminals. Neurons often have highly elaborate branches innervating multiple targets, but how protein or membrane cargos navigate through branch junctions to specific branch targets is unknown. Here, we demonstrate that anterograde transport of membrane vesicles through axonal branch junctions is highly selective, which is influenced by branch length and more strongly by growth cone motility. Using an optogenetic tool, we demonstrate that signaling from the growth cone can rapidly direct transport through branch junctions. We further demonstrate that such transport selectivity is differentially regulated for different vesicles and mediated by the KIF1/kinesin-3 family motors. We propose that this transport regulation through branch junctions could broadly impact neuronal development, function, and regeneration.

Recommended Citation

Tymanskyj, Stephen R.; Curran, Bridget M.; and Ma, Le, "Selective axonal transport through branch junctions is directed by growth cone signaling and mediated by KIF1/kinesin-3 motors." (2022). Department of Neuroscience Faculty Papers. Paper 67.
https://jdc.jefferson.edu/department_neuroscience/67

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Language

English