Tao Wang, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Zhuo Liu, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Shuiming Guo, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Licheng Wu, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Mingchao Li, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Jun Yang, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Ruibao Chen, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Hua Xu, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Shaoxin Cai, Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Hui Chen, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Weiyong Li, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Liang Wang, Department of Radiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Zhiquan Hu, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Qianyuan Zhuang, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Shaohua Xu, Kimmel Cancer Center, Department of Cancer Biology, Thomas Jefferson UniversityFollow
Liping Wang, Kimmel Cancer Center, Department of Cancer Biology, Thomas Jefferson UniversityFollow
Jihong Liu, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Zhangqun Ye, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow
Jun-Yuan Ji, Department of Molecular and Cellular Medicine, College of Medicine, Texas A and M University Health Science CenterFollow
Chenguang Wang, Kimmel Cancer Center, Department of Cancer Biology, Thomas Jefferson UniversityFollow
Ke Chen, Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyFollow

Document Type

Article

Publication Date

6-15-2014

Comments

This article has been peer reviewed. It was published in: Oncotarget. Volume 5, Issue 11, 2014, Pages 3611-3621.


The published version is available at http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=1968. Copyright © Wang et. al.

Abstract

Prostate cancer at advanced stages including metastatic and castration-resistant cancer remains incurable due to the lack of effective therapies. The CAMK2N1 gene, cloned and characterized as an inhibitor of CaMKII (calcium/calmodulin-dependent protein kinase II), has been shown to affect tumorigenesis and tumor growth. However, it is still unknown whether CAMK2N1 plays a role in prostate cancer development. We first examined the protein and mRNA levels of CAMK2N1 and observed a significant decrease in human prostate cancers comparing to normal prostate tissues. Re-expression of CAMK2N1 in prostate cancer cells reduced cellular proliferation, arrested cells in G0/G1 phases, and induced apoptotic cell death accompanied by down-regulation of IGF-1, ErbB2, and VEGF downstream kinases PI3K/AKT, as well as the MEK/ERK-mediated signaling pathways. Conversely, knockdown of CAMK2N1 had a significant opposite effects on these phenotypes. Our analyses suggest that CAMK2N1 plays a tumor suppressive role in prostate cancer cells. Reduced CAMK2N1 expression correlates to human prostate cancer progression and predicts poor clinical outcome, indicating that CAMK2N1 may serve as a biomarker. The inhibition of tumor growth by expressing CAMK2N1 established a role of CAMK2N1 as a therapeutic target.

PubMed ID

25003983

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