Tissue-specific regulation of mouse microRNA genes in endoderm-derived tissues.

Authors

Yan Gao, University of Pennsylvania School of MedicineFollow
Jonathan Schug, University of Pennsylvania School of MedicineFollow
Lindsay B McKenna, University of Pennsylvania School of MedicineFollow
John Le Lay, University of Pennsylvania School of Medicine
Klaus H Kaestner, University of Pennsylvania School of MedicineFollow
Linda E Greenbaum, Thomas Jefferson UniversityFollow

Document Type

Article

Publication Date

1-1-2011

Comments

This article has been peer reviewed. It was published in: Nucleic acids research.

Volume 39, Issue 2, January 2011, Pages 454-63.

The published version is available at DOI: 10.1093/nar/gkq782. Copyright © Oxford Journals.

Abstract

MicroRNAs fine-tune the activity of hundreds of protein-coding genes. The identification of tissue-specific microRNAs and their promoters has been constrained by the limited sensitivity of prior microRNA quantification methods. Here, we determine the entire microRNAome of three endoderm-derived tissues, liver, jejunum and pancreas, using ultra-high throughput sequencing. Although many microRNA genes are expressed at comparable levels, 162 microRNAs exhibited striking tissue-specificity. After mapping the putative promoters for these microRNA genes using H3K4me3 histone occupancy, we analyzed the regulatory modules of 63 microRNAs differentially expressed between liver and jejunum or pancreas. We determined that the same transcriptional regulatory mechanisms govern tissue-specific gene expression of both mRNA and microRNA encoding genes in mammals.

Recommended Citation

Gao, Yan; Schug, Jonathan; McKenna, Lindsay B; Le Lay, John; Kaestner, Klaus H; and Greenbaum, Linda E, "Tissue-specific regulation of mouse microRNA genes in endoderm-derived tissues." (2011). Department of Cancer Biology Faculty Papers. Paper 45.
https://jdc.jefferson.edu/cbfp/45

PubMed ID

20843784