Document Type
Article
Publication Date
1-1-2013
Abstract
BACKGROUND: Validation of physiologic miRNA targets has been met with significant challenges. We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs.
RESULTS: miRNA recruitment to the RISC is highly dynamic, changing more than five-fold for several miRNAs. miRNA recruitment to the RISC did not correlate with changes in overall miRNA expression for these dynamically recruited miRNAs, emphasizing the necessity to determine miRNA recruitment to the RISC in order to fully assess the impact of miRNA regulation. We incorporated RNA-seq quantification of total mRNA to identify expression-weighted Ago footprints, and developed a microRNA regulatory element (MRE) prediction algorithm that represents a greater than 20-fold refinement over computational methods alone. These high confidence MREs were used to generate candidate 'competing endogenous RNA' (ceRNA) networks.
CONCLUSION: HITS-CLIP analysis provide novel insights into global miRNA:mRNA relationships in the regenerating liver.
Recommended Citation
Schug, Jonathan; McKenna, Lindsay B; Walton, Gabriel; Hand, Nicholas; Mukherjee, Sarmistha; Essuman, Kow; Shi, Zhongjie; Gao, Yan; Markley, Karen; Nakagawa, Momo; Kameswaran, Vasumathi; Vourekas, Anastassios; Friedman, Joshua R; Kaestner, Klaus H; and Greenbaum, Linda E, "Dynamic recruitment of microRNAs to their mRNA targets in the regenerating liver." (2013). Department of Cancer Biology Faculty Papers. Paper 34.
https://jdc.jefferson.edu/cbfp/34
PubMed ID
23597149
Comments
This article has been peer reviewed. It was published in: BMC Genomics.
2013 Apr 18;14:264.
The published version is available at DOI: 10.1186/1471-2164-14-264. Copyright © BioMed Central Ltd.