Document Type
Article
Publication Date
11-20-2014
Abstract
Long non-coding RNAs (lncRNAs) represent an emerging layer of cancer biology, contributing to tumor proliferation, invasion, and metastasis. Here, we describe a role for the oncogenic lncRNA PCAT-1 in prostate cancer proliferation through cMyc. We find that PCAT-1-mediated proliferation is dependent on cMyc protein stabilization, and using expression profiling, we observed that cMyc is required for a subset of PCAT-1-induced expression changes. The PCAT-1-cMyc relationship is mediated through the post-transcriptional activity of the MYC 3' untranslated region, and we characterize a role for PCAT-1 in the disruption of MYC-targeting microRNAs. To further elucidate a role for post-transcriptional regulation, we demonstrate that targeting PCAT-1 with miR-3667-3p, which does not target MYC, is able to reverse the stabilization of cMyc by PCAT-1. This work establishes a basis for the oncogenic role of PCAT-1 in cancer cell proliferation and is the first study to implicate lncRNAs in the regulation of cMyc in prostate cancer.
Recommended Citation
Prensner, John R.; Chen, Wei; Han, Sumin; Iyer, Matthew K.; Cao, Qi; Kothari, Vishal; Evans, Joseph R.; Knudsen, Karen E.; Paulsen, Michelle T.; Ljungman, Mats; Lawrence, Theodore S.; Chinnaiyan, Arul M.; and Feng, Felix Y., "The long non-coding RNA PCAT-1 promotes prostate cancer cell proliferation through cMyc." (2014). Department of Cancer Biology Faculty Papers. Paper 100.
https://jdc.jefferson.edu/cbfp/100
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
PubMed ID
25425964
Comments
This article has been peer reviewed. It is the author’s final published version in Neoplasia
Volume 16, Issue 11, November 2014, Pages 900-908
The published version is available at DOI: 10.1016/j.neo.2014.09.001. Copyright © Prensner et al.