Global analysis of SUMO chain function reveals multiple roles in chromatin regulation.

Authors

• Tharan Srikumar, Ontario Cancer Institute, University Health Network; Department of Medical Biophysics, University of Toronto
• Megan C Lewicki, Ontario Cancer Institute, University Health Network; Department of Medical Biophysics, University of Toronto
• Michael Costanzo, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto
• Johnny M Tkach, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto; Department of Biochemistry, University of Toronto
• Harm van Bakel, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto; Banting and Best Department of Medical Research, University of Toronto
• Kyle Tsui, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto; Department of Pharmaceutical Sciences, University of Toronto
• Erica S Johnson, Department of Biochemistry and Molecular Biology, Thomas Jefferson UniversityFollow
• Grant W Brown, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto; Department of Biochemistry, University of Toronto
• Brenda J Andrews, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto; Banting and Best Department of Medical Research, University of Toronto; Department of Molecular Genetics, University of Toronto
• Charles Boone, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto; Banting and Best Department of Medical Research, University of Toronto; Department of Molecular Genetics, University of Toronto
• Guri Giaever, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto; Department of Pharmaceutical Sciences, University of Toronto; Department of Molecular Genetics, University of Toronto
• Corey Nislow, Terence Donnelly Center for Cellular and Biomolecular Research, University of Toronto; Banting and Best Department of Medical Research, University of Toronto; Department of Molecular Genetics, University of Toronto
• Brian Raught, Ontario Cancer Institute, University Health Network; Department of Medical Biophysics, University of Toronto

Document Type

Article

Publication Date

4-1-2013

Comments

This article has been peer reviewed. It was published in: Journal of Cell Biology.
Volume 201, Issue 1, April 2013, Pages 145-163.
The published version is available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613684/. DOI: 10.1083/jcb.201210019

Copyright © 2013 Srikumar et al.

Abstract

Like ubiquitin, the small ubiquitin-related modifier (SUMO) proteins can form oligomeric "chains," but the biological functions of these superstructures are not well understood. Here, we created mutant yeast strains unable to synthesize SUMO chains (smt3(allR)) and subjected them to high-content microscopic screening, synthetic genetic array (SGA) analysis, and high-density transcript profiling to perform the first global analysis of SUMO chain function. This comprehensive assessment identified 144 proteins with altered localization or intensity in smt3(allR) cells, 149 synthetic genetic interactions, and 225 mRNA transcripts (primarily consisting of stress- and nutrient-response genes) that displayed a >1.5-fold increase in expression levels. This information-rich resource strongly implicates SUMO chains in the regulation of chromatin. Indeed, using several different approaches, we demonstrate that SUMO chains are required for the maintenance of normal higher-order chromatin structure and transcriptional repression of environmental stress response genes in budding yeast.

Recommended Citation

Srikumar, Tharan; Lewicki, Megan C; Costanzo, Michael; Tkach, Johnny M; van Bakel, Harm; Tsui, Kyle; Johnson, Erica S; Brown, Grant W; Andrews, Brenda J; Boone, Charles; Giaever, Guri; Nislow, Corey; and Raught, Brian, "Global analysis of SUMO chain function reveals multiple roles in chromatin regulation." (2013). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 67.
https://jdc.jefferson.edu/bmpfp/67

PubMed ID

23547032