Ribosome recycling, the last step in translation, is now accepted as an essential process for prokaryotes. In 2005, three laboratories showed that ribosome-recycling factor (RRF) and elongation factor G (EF-G) cause dissociation of ribosomes into subunits, solving the long-standing problem of how this essential step of translation occurs. However, there remains ongoing controversy regarding the other actions of RRF and EF-G during ribosome recycling. We propose that the available data are consistent with the notion that RRF and EF-G not only split ribosomes into subunits but also participate directly in the release of deacylated tRNA and mRNA for the next round of translation.
Hirokawa, Go; Demeshkina, Natalia; Iwakura, Nobuhiro; Kaji, Hideko; and Kaji, Akira, "The ribosome-recycling step: consensus or controversy?" (2006). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 6.