Document Type
Article
Publication Date
6-14-2024
Abstract
Smk1 is a MAPK homolog in the yeast Saccharomyces cerevisiae that controls the postmeiotic program of spore wall assembly. During this program, haploid cells are surrounded by a layer of mannan and then a layer of glucan. These inner layers of the spore wall resemble the vegetative cell wall. Next, the outer layers consisting of chitin/chitosan and then dityrosine are assembled. The outer layers are spore-specific and provide protection against environmental stressors. Smk1 is required for the proper assembly of spore walls. However, the protective properties of the outer layers have limited our understanding of how Smk1 controls this morphogenetic program. Mutants lacking the chitin deacetylases, Cda1 and Cda2, form spores that lack the outer layers of the spore wall. In this study, cda1,2∆ cells were used to demonstrate that Smk1 promotes deposition of the glucan layer of the spore wall through the partially redundant glucan synthases Gsc2 and Fks3. Although Gsc2 is localized to sites of spore wall assembly in the wild type, it is mislocalized in the mother cell cytoplasm in the smk1∆ mutant. These findings suggest that Smk1 controls assembly of the spore wall by regulating the localization of Gsc2 during sporogenesis.
Recommended Citation
Lee-Soety, Julia Y.; Resch, Gwendolyn; Rimal, Abhimannyu; Johnson, Erica S.; Benway, Jonathan; and Winter, Edward, "The MAPK Homolog, Smk1, Promotes Assembly of the Glucan Layer of the Spore Wall in S. cerevisiae" (2024). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 287.
https://jdc.jefferson.edu/bmpfp/287
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
PubMed ID
38874213
Language
English
Comments
This article is the author’s final published version in Yeast, Volume 41, Issue 7, July 2024, Pages 448-457.
The published version is available at https://doi.org/10.1002/yea.3967. Copyright © 2024 The Author(s). Yeast published by John Wiley & Sons Ltd.
Publication made possible in part by support through a transformative agreement between Thomas Jefferson University and the publisher.