Differentiating PC12 Cells to Evaluate Neurite Densities Through Live-Cell Imaging

Authors

Jordyn Karliner, Thomas Jefferson UniversityFollow
Diane E Merry, Thomas Jefferson UniversityFollow

Document Type

Article

Publication Date

1-4-2023

Comments

This article is the author’s final published version in STAR Protocols, Volume 4, Issue 1, January 2023, Article number 101993.

The published version is available at https://doi.org/10.1016/j.xpro.2022.101993. Copyright © Karliner and Merry.

Abstract

Although PC12 cells are a valuable tool in neuroscience research, previously published PC12 cell differentiation techniques fail to consider the variability in differentiation rates between different PC12 cell strains and clonal variants. Here, we present a comprehensive protocol to differentiate PC12 cells into equivalent neurite densities through live-cell imaging for morphological, immunocytochemical, and biochemical analyses. We detail steps on optimized substrate coating, plating techniques, culture media, validation steps, and quantification techniques.

Recommended Citation

Karliner, Jordyn and Merry, Diane E, "Differentiating PC12 Cells to Evaluate Neurite Densities Through Live-Cell Imaging" (2023). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 232.
https://jdc.jefferson.edu/bmpfp/232

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

PubMed ID

36602900

Language

English