Document Type

Article

Publication Date

11-17-2010

Comments

This article has been peer reviewed. It is the authors' final version prior to publication in Bioconjugate Chemistry Volume 21, Issue 11, November 2010, Pages 1978-1986. The published version is available at DOI: 10.1021/bc100136e. Copyright © American Chemical Society

Abstract

Infections are a devastating complication of titanium alloy orthopedic implants. Current therapy includes antibiotic-impregnated bone cement and antibiotic-containing coatings. We hypothesized that daptomycin, a Gram-positive peptide antibiotic, could prevent bacterial colonization on titanium alloy surfaces if covalently bonded via a flexible, hydrophilic spacer. We designed and synthesized a series of daptomycin conjugates for bonding to the surface of 1.0 cm² Ti6Al4V foils through bisphosphonate groups, reaching a maximum yield of 180 pmol/cm². Daptomycin-bonded foils killed 53 ± 5% of a high challenge dose of 3 × 10⁵ cfu Staphylococcus aureus ATCC 29213.

PubMed ID

20949909

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