Structural basis of mitochondrial transcription.

Authors

Hauke S. Hillen, Max Planck Institute for Biophysical Chemistry
Dmitry Temiakov, Thomas Jefferson University; Rowan UniversityFollow
Patrick Cramer, Max Planck Institute for Biophysical Chemistry

Document Type

Article

Publication Date

9-1-2018

Comments

This article has been peer reviewed. It is the authors' final version prior to publication in Nature Structural and Molecular Biology, Volume 25, Issue 9, September 2018, Pages 754-765.

The published version is available at https://doi.org/10.1038/s41594-018-0122-9. Copyright © Springer Nature America, Inc.

Abstract

The mitochondrial genome is transcribed by a single-subunit DNA-dependent RNA polymerase (mtRNAP) and its auxiliary factors. Structural studies have elucidated how mtRNAP cooperates with its dedicated transcription factors to direct RNA synthesis: initiation factors TFAM and TFB2M assist in promoter-DNA binding and opening by mtRNAP while the elongation factor TEFM increases polymerase processivity to the levels required for synthesis of long polycistronic mtRNA transcripts. Here, we review the emerging body of structural and functional studies of human mitochondrial transcription, provide a molecular movie that can be used for teaching purposes and discuss the open questions to guide future directions of investigation.

Recommended Citation

Hillen, Hauke S.; Temiakov, Dmitry; and Cramer, Patrick, "Structural basis of mitochondrial transcription." (2018). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 158.
https://jdc.jefferson.edu/bmpfp/158

PubMed ID

30190598

Language

English