Crosstalk between Desmoglein 2 and Patched 1 accelerates chemical-induced skin tumorigenesis.

Authors

Donna M Brennan-Crispi, Department of Dermatology and Cutaneous Biology, Department of Biochemistry and Molecular Biology, Sidney Kimmel Cancer Center, Thomas Jefferson UniversityFollow
Claudia Hossain, Department of Dermatology and Cutaneous Biology, Thomas Jefferson UniversityFollow
Joya Sahu, Department of Dermatology and Cutaneous Biology, Thomas Jefferson UniversityFollow
Mary Brady, Department of Dermatology and Cutaneous Biology, Thomas Jefferson UniversityFollow
Natalia A Riobo, Sidney Kimmel Cancer Center, Department of Biochemistry and Molecular Biology, Thomas Jefferson UniversityFollow
M G Mahoney, Sidney Kimmel Cancer Center, Thomas Jefferson UniversityFollow

Document Type

Article

Publication Date

4-20-2015

Comments

This article has been peer reviewed. It was published in: Oncotarget.

Volume 6, Issue 11, 2015, Pages 8593-8605.

The published version is available at http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=3309

Copyright © 2015 the authors

Abstract

Aberrant activation of Hedgehog (Hh) signaling is causative of BCCs and has been associated with a fraction of SCCs. Desmoglein 2 (Dsg2) is an adhesion protein that is upregulated in many cancers and overexpression of Dsg2 in the epidermis renders mice more susceptible to squamous-derived neoplasia. Here we examined a potential crosstalk between Dsg2 and Hh signaling in skin tumorigenesis. Our findings show that Dsg2 modulates Gli1 expression, in vitro and in vivo. Ectopic expression of Dsg2 on Ptc1+/lacZ background enhanced epidermal proliferation and interfollicular activation of the Hh pathway. Furthermore, in response to DMBA/TPA, the Dsg2/Ptc1+/lacZ mice developed squamous lessons earlier than the WT, Ptc1+/lacZ, and Inv-Dsg2 littermates. Additionally, DMBA/TPA induced BCC formation in all mice harboring the Ptc1+/lacZ gene and the presence of Dsg2 in Dsg2/Ptc1+/lacZ mice doubled the BCC tumor burden. Reporter analysis revealed activation of the Hh pathway in the BCC tumors. However, in the SCCs we observed Hh activity only in the underlying dermis of the tumors. Furthermore, Dsg2/Ptc1+/lacZ mice demonstrated enhanced MEK/Erk1/2 activation within the tumors and expression of Shh in the dermis. In summary, our results demonstrate that Dsg2 modulates Hh signaling, and this synergy may accelerate skin tumor development by different mechanisms.

Recommended Citation

Brennan-Crispi, Donna M; Hossain, Claudia; Sahu, Joya; Brady, Mary; Riobo, Natalia A; and Mahoney, M G, "Crosstalk between Desmoglein 2 and Patched 1 accelerates chemical-induced skin tumorigenesis." (2015). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 86.
https://jdc.jefferson.edu/bmpfp/86

PubMed ID

25871385