Early Prediction of the Breast Cancer Response to Neoadjuvant Chemotherapy Based on Intratumoral Interstitial Fluid Pressure Using 3-D Subharmonic-Aided Pressure Estimation

Authors

• Sung In Choi, Thomas Jefferson UniversityFollow
• Basak Dogan
• Corinne E. Wessner, Thomas Jefferson UniversityFollow
• Jessica H. Porembka
• Priscilla Machado, Thomas Jefferson UniversityFollow
• B. Bersu Ozcan
• Nisha Unni
• Maysa Abu-Khalaf
• Kenneth Hoyt
• Flemming Forsberg, Thomas Jefferson UniversityFollow
• Kibo Nam, Thomas Jefferson UniversityFollow

Document Type

Article

Publication Date

10-1-2026

Comments

This article is the author's final published version in Ultrasound in Medicine and Biology, Volume 52, Issue 10, October 2026, Pages 2260-2268.

The published version is available at https://doi.org/10.1016/j.ultrasmedbio.2026.06.023. Copyright © 2026 The Authors.

Abstract

Elevated intratumoral interstitial fluid pressure (IFP) may negatively impact the efficacy of neoadjuvant chemotherapy (NAC). This study aimed to predict the breast cancer response to NAC based on intratumoral IFP using 3D contrast-enhanced ultrasound (CEUS) with subharmonic-aided pressure estimation (SHAPE). This prospective study enrolled breast cancer patients undergoing NAC from two clinical sites. 3D CEUS was performed at 0%, 10%, and 30% of NAC completion using a GE Logiq E9 with Definity. The pressure gradients between the tumor and adjacent normal tissue were estimated by SHAPE, utilizing the inverse linear relationship between the ultrasound contrast agent’s subharmonic signal amplitude and hydrostatic pressure. The obtained SHAPE gradients were evaluated for the prediction of pathological complete response (pCR) and radiological response (RR, tumor volume reduction ≥90%), using t-test and receiver operating characteristic curve analysis. Additionally, the effect of incorporating tumor volume into the SHAPE gradient was evaluated. This study included thirty-seven patients. SHAPE gradients were significantly different between pCR and non-pCR at 0% (p = 0.03), 10% (p = 0.03), and 30% (p = 0.001) completion of NAC, and between RR and non-RR at 30% (p = 0.007) completion of NAC. The optimal predictive performance for SHAPE was achieved at 30% NAC completion, yielding area under the curve (AUC) values of 0.85 for pCR and 0.79 for RR. By combining the tumor volume-based parameters and SHAPE gradient, AUCs were 0.98 for pCR and 0.84 for RR predictions at 30% NAC completion. SHAPE gradient, independently or combined with tumor volume may aid in the early prediction of breast cancer NAC response.

Recommended Citation

Choi, Sung In; Dogan, Basak; Wessner, Corinne E.; Porembka, Jessica H.; Machado, Priscilla; Ozcan, B. Bersu; Unni, Nisha; Abu-Khalaf, Maysa; Hoyt, Kenneth; Forsberg, Flemming; and Nam, Kibo, "Early Prediction of the Breast Cancer Response to Neoadjuvant Chemotherapy Based on Intratumoral Interstitial Fluid Pressure Using 3-D Subharmonic-Aided Pressure Estimation" (2026). Department of Radiology Faculty Papers. Paper 199.
https://jdc.jefferson.edu/radiologyfp/199

Creative Commons License

This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

PubMed ID

42471280

Language

English