Document Type
Article
Publication Date
1-1-2026
Abstract
BACKGROUND: Stroke leads to complex chronic structural and functional brain changes that specifically affect motor outcomes. The brain predicted age difference (PAD) has emerged as a sensitive biomarker of both sensorimotor and cognitive function after stroke. Our previous study showed a higher global brain PAD associated with poorer motor function after stroke. However, the association between local stroke lesion load, regional brain age, and motor impairment is unclear. This study aimed to investigate the associations between focal lesion damage, regional brain PAD in both hemispheres, and motor outcomes in chronic stroke, and to identify key predictors of motor impairment.
METHODS: In this multicohort, retrospective, observational study, we included individuals with chronic unilateral stroke (>180 days post stroke) from the ENIGMA Stroke Recovery Working Group dataset and used individuals from the UK Biobank cohort to train the regional brain age prediction model. Structural T1-weighted MRI scans were used to estimate regional brain PAD in 18 predefined functional subregions via a graph convolutional network algorithm. Lesion load for each region was calculated on the basis of lesion overlap. Linear mixed-effects models assessed associations between lesion size, local lesion load, and regional brain PAD. Machine learning classifiers predicted motor outcomes using lesion loads and regional brain PADs. Structural equation modelling examined directional relationships among corticospinal tract lesion load, ipsilesional brain PAD, motor outcomes, and contralesional brain PAD.
FINDINGS: We included 501 individuals from the ENIGMA Stroke Recovery Working Group dataset (34 cohorts in eight countries) and 17 791 individuals from the UK Biobank dataset. Larger total lesion size was positively associated with higher ipsilesional regional brain PADs (older brain age) across most regions (β=0·5420 to 0·9458 across significantly correlated regions, false discovery rate [FDR]-corrected p< 0·05), and with lower brain PAD in the contralesional ventral attention and language network region (β=-0·3747, 95% CI -0·6961 to -0·0534, FDR-corrected p< 0·05). Higher local lesion loads showed similar patterns. Specifically, lesion load in the salience network significantly influenced regional brain PADs across both hemispheres. Machine learning models identified corticospinal tract lesion load (adjusted mean difference -0·0905, 95% CI -0·1221 to -0·0589, p< 0·0001), salience network lesion load (-0·0632, -0·0906 to -0·0358, p< 0·0001), and regional brain PAD in the contralesional frontoparietal network (0·9939, 0·4929 to 1·4950, p=0·0001) as the top three predictors of motor outcomes. Structural equation modelling revealed that higher corticospinal tract lesion load was associated with poorer motor outcomes (β=-0·355, 95% CI -0·446 to -0·267, p< 0·0001), which were further linked to younger contralesional brain age (0·204, 0·111 to 0·295, p< 0·0001), suggesting that severe motor impairment is linked to compensatory decreases in contralesional brain age.
INTERPRETATION: Our findings reveal that larger stroke lesions are associated with accelerated ageing in the ipsilesional hemisphere and paradoxically decelerated brain ageing in the contralesional hemisphere, suggesting compensatory neural mechanisms. Assessing regional brain age might serve as a biomarker for neuroplasticity and inform targeted interventions to enhance motor recovery after stroke.
FUNDING: US National Institutes of Health.
Recommended Citation
Park, Gilsoon; Khan, Mahir; Andrushko, Justin; Banaj, Nerisa; Borich, Michael; Boyd, Lara; Brodtmann, Amy; Brown, Truman; Buetefisch, Cathrin; Conforto, Adriana; Cramer, Steven; Dimyan, Michael; Domin, Martin; Donnelly, Miranda; Egorova-Brumley, Natalia; Ermer, Elsa; Feng, Wuwei; Geranmayeh, Fatemeh; Hanlon, Colleen; Hordacre, Brenton; Jahanshad, Neda; Kautz, Steven; Khlif, Mohamed Salah; Liu, Jingchun; Lotze, Martin; MacIntosh, Bradley; Mohamed, Feroze; Nordvik, Jan; Piras, Fabrizio; Revill, Kate; Robertson, Andrew; Schranz, Christian; Schweighofer, Nicolas; Seo, Na Jin; Soekadar, Surjo; Srivastava, Shraddha; Tavenner, Bethany; Thielman, Gregory; Thomopoulos, Sophia; Vecchio, Daniela; Werden, Emilio; Westlye, Lars; Winstein, Carolee; Wittenberg, George; Ferris, Jennifer; Yu, Chunshui; Thompson, Paul; Liew, Sook-Lei; and Kim, Hosung, "Associations Between Contralesional Neuroplasticity and Motor Impairment Through Deep Learning-Derived MRI Regional Brain Age in Chronic Stroke (Enigma): A Multicohort, Retrospective, Observational Study" (2026). Department of Radiology Faculty Papers. Paper 192.
https://jdc.jefferson.edu/radiologyfp/192
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
PubMed ID
41577565
Language
English
Included in
Cardiovascular Diseases Commons, Musculoskeletal, Neural, and Ocular Physiology Commons, Nervous System Diseases Commons, Radiology Commons
Comments
This article is the author’s final published version in The Lancet. Digital health, Volume 8, Issue 1, 2026, Article number 100942.
The published version is available at https://doi.org/10.1016/j.landig.2025.100942. Copyright © 2025 The Author(s).