Cannabinoid receptor type-2 modulates nociceptive signaling molecules in a model of post-concussion headache

Authors

Jarred M. Stratton, Department of Neurosurgery, Thomas Jefferson UniversityFollow
Lan Cheng, Center for Translational Medicine, Department of Medicine, Thomas Jefferson UniversityFollow
Ashley L. Tyburski, Department of Neurosurgery, Thomas Jefferson UniversityFollow
Jessica Perino, Department of Neurosurgery, Thomas Jefferson UniversityFollow
Melanie B Elliott, Department of Neurological Surgery, Thomas Jefferson UniversityFollow

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Description

Abstract

The goal of this study was to investigate the role of the cannabinoid receptor type-2 (CB₂R) in the trigeminal pain pathway in a model of post-concussion headache. Sprague Dawley rats were randomized to receive either a repeated mild closed head injury (CHI) or served as incision controls. Changes in CGRP, nNOS, and IBA-1 were assessed in the trigeminal nucleus caudalis (TNC) and trigeminal ganglia via IHC. A subset of CHI rats received either a cannabinoid receptor type 2 (CB₂R) anti-inflammatory agonist (JWH133), an NSAID (Ketorolac), or vehicle and underwent von Frey testing for trigeminal allodynia. An in vitro brain slice study was performed on TNC and cerebrum slices incubated with capsaicin, capsaicin plus a or media control solutions for 24hrs; CGRP and PGE₂ were assessed via ELISA. Repeated CHI showed increases in CGRP and PGE₂, and altered nNOS and IBA-1 immunoreactivity in the trigeminal ganglia and TNC, respectively. JWH-133 blocked capsaicin-induced increases in CGRP and PGE2 in the TNC and cerebrum slices. Findings show the CB₂R modulates trigeminal pain in a model of concussion, although the mechanisms eliciting analgesia warrant a more in depth investigation.

Poster presented at: American Headache Society in San Diego California.

Publication Date

6-20-2016

Keywords

Cannabinoid receptor type-2 modulates nociceptive signaling molecules in a model of post-concussion headache, Department of Neurosurgery, Thomas Jefferson University, poster

Disciplines

Surgery

Comments

Poster presented at: 2016 Sigma XI Student Research Day.

Recommended Citation

Stratton, Jarred M.; Cheng, Lan; Tyburski, Ashley L.; Perino, Jessica; and Elliott, Melanie B, "Cannabinoid receptor type-2 modulates nociceptive signaling molecules in a model of post-concussion headache" (2016). Department of Neurosurgery Posters. 4.
https://jdc.jefferson.edu/neurosurgeryposters/4