Onchocerciasis remains a debilitating neglected tropical disease. Due to the many challenges of current control methods, an effective vaccine against the causative agent Onchocerca volvulus is urgently needed. Mice and cynomolgus macaque non-human primates (NHPs) were immunized with a vaccine consisting of a fusion of two O. volvulus protein antigens, Ov-103 and Ov-RAL-2 (Ov-FUS-1), and three different adjuvants: Advax-CpG, alum, and AlT4. All vaccine formulations induced high antigen-specific IgG titers in both mice and NHPs. Challenging mice with O. volvulus L3 contained within subcutaneous diffusion chambers demonstrated that Ov-FUS-1/Advax-CpG-immunized animals developed protective immunity, durable for at least 11 weeks. Passive transfer of sera, collected at several time points, from both mice and NHPs immunized with Ov-FUS-1/Advax-CpG transferred protection to naïve mice. These results demonstrate that Ov-FUS-1 with the adjuvant Advax-CpG induces durable protective immunity against O. volvulus in mice and NHPs that is mediated by vaccine-induced humoral factors.
Ryan, Nathan M.; Hess, Jessica A.; Robertson, Erica J.; Tricoche, Nancy; Turner, Cheri; Davis, Jenn; Petrovsky, Nikolai; Ferguson, Melissa; Rinaldi, William J.; Wong, Valerie M.; Shimada, Ayako; Zhan, Bin; Bottazzi, Maria Elena; Makepece, Benjamin L.; Gray, Sean A.; Carter, Darrick; Lustigman, Sara; and Abraham, David, "Adjuvanted Fusion Protein Vaccine Induces Durable Immunity to Onchocerca volvulus in Mice and Non-Human Primates" (2023). Department of Microbiology and Immunology Faculty Papers. Paper 177.
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