Document Type
Article
Publication Date
9-1-2014
Abstract
Vaccination of Mongolian gerbils with Brugia malayi cysteine protease inhibitor-2 in which the amino acid Asn66 was mutated to Lys66 (Bm-CPI-2M) resulted in reduced parasite numbers of 48.6% and 48.0% at 42 and 90 days p.i. with B. malayi L3s. Fertility of female worms was also affected at 90 days p.i. In vitro killing of L3s observed in the presence of gerbil peritoneal exudate cells and anti-Bm-CPI-2M sera suggests antibody-dependent cell-mediated cytotoxicity as a putative protective mechanism. These observations suggest that Bm-CPI-2M is a promising prophylactic and anti-fecundity vaccine candidate.
Recommended Citation
Arumugam, Sridhar; Wei, Junfei; Ward, Danielle; Abraham, David; Lustigman, Sara; Zhan, Bin; and Klei, Thomas R., "Vaccination with a genetically modified Brugia malayi cysteine protease inhibitor-2 reduces adult parasite numbers and affects the fertility of female worms following a subcutaneous challenge of Mongolian gerbils (Meriones unguiculatus) with B. malayi infective larvae." (2014). Department of Microbiology and Immunology Faculty Papers. Paper 146.
https://jdc.jefferson.edu/mifp/146
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
PubMed ID
24929131
Language
English
Comments
This article is the authors' final version prior to publication in International Journal of Parasitology, Volume 44, Issue 10, September 2014, Pages 675-679.
The published version is available at https://doi.org/10.1016/j.ijpara.2014.05.003. Copyright © Arumugam et al.