Document Type
Article
Publication Date
10-8-2025
Abstract
BACKGROUND AND OBJECTIVES: Retinal optical coherence tomography (OCT) in rodent models has been used to longitudinally image retinal changes, to define end points for more costly or time-consuming experiments, and to better understand the pathophysiology underlying OCT findings in human diseases. No standardization of rodent OCT reporting currently exists. Here, we aim to establish consensus recommendation for reporting results from retinal OCT studies in rodents.
METHODS: Initial recommendations were developed based on the APOSTEL criteria for quantitative OCT reporting in humans by a core team. Using a modified Delphi process, an expert panel of rodent OCT researchers (N = 31) and the wider scientific community discussed, refined, and voted on these initial recommendations. The list of recommendations was then revised and approved by the expert panel.
RESULTS: The final 7-point checklist includes reporting recommendations regarding the study protocol, OCT device, acquisition settings and modifications, scanning protocol, funduscopic imaging, postacquisition data selection and image data analyses, and qualitative and quantitative results. With a median agreement score of 3 or 4 out of 4, the scientific community agreed with these recommendations. After revisions, the expert panel accepted the final recommendations.
DISCUSSION: The Advised Protocol for OCT Study Terminology and Elements for reporting OCT studies in rodents (APOSTEL-R) originates from an expert consensus. They will provide guidance throughout the experimental process and will contribute to the standardization and quality improvement of preclinical OCT studies.
Recommended Citation
Oertel, Frederike Cosima; Cabrera Debuc, Delia; Calabresi, Peter A.; Chen, Mei; Cordano, Christian; Dietrich, Michael; Feltgen, Nicolas; Gramlich, Oliver; Green, Ari J.; Groh, Janos; Huang, Su-Chun; Knier, Benjamin; Korn, Thomas; Leocani, Letizia; Loewenstein, Anat; van Oterendorp, Christian; Peto, Tunde; Schippling, Sven; Schmetterer, Leopold; Schmitz-Valckenberg, Steffen; Seeliger, Mathias W.; Shindler, Kenneth S.; Schuman, Joel; Sindi, Mustafa; Tufail, Adnan; Wang, Jui-Kai; Wolf, Sebastian; You, Yuyi; Zinkernagel, Martin; Lagrèze, Wolf; and Albrecht, Philipp, "APOSTEL-R Recommendations for Reporting Retinal Optical Coherence Tomography Studies in Rodents" (2025). Department of Medicine Faculty Papers. Paper 517.
https://jdc.jefferson.edu/medfp/517
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.
PubMed ID
41061181
Language
English
Comments
This article is the author’s final published version in Neurology(R) neuroimmunology & neuroinflammation, Volume 12, Issue 6, 2025, Article number e200489.
The published version is available at https://doi.org/10.1212/NXI.0000000000200489. Copyright © 2025 The Author(s).