Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-alpha-acetyltransferase.

Authors

Thomas Arnesen, Department of Molecular Biology, University of Bergen, N-5020 Bergen, Norway, Department of Surgical Sciences, Haukeland University Hospital, N-5021 Bergen, NorwayFollow
Matthew J Betts, Computational Biology Unit, BCCS, University of Bergen, N-5020 Bergen, NorwayFollow
Frédéric Pendino, Department of Molecular Biology, University of Bergen, N-5020 Bergen, NorwayFollow
David A Liberles, Computational Biology Unit, BCCS, University of Bergen, N-5020 Bergen, NorwayFollow
Dave Anderson, Institute of Molecular Biology, University of Oregon, Eugene, OR 97403-1229, USAFollow
Jaime Caro, Department of Medicine, Thomas Jefferson University, Philadelphia, PA19107, USAFollow
Xianguo Kong, Department of Medicine, Thomas Jefferson University, Philadelphia, PA19107, USAFollow
Jan E Varhaug, Department of Surgical Sciences, Haukeland University Hospital, N-5021 Bergen, NorwayFollow
Johan R Lillehaug, Department of Molecular Biology, University of Bergen, N-5020 Bergen, NorwayFollow

Document Type

Article

Publication Date

1-1-2006

Comments

This article has been peer reviewed and is published in BMC Biochemistry Volume 7, 25 April 2006, Article number 13. The published version is available at DOI: 10.1186/1471-2091-7-13. Copyright © BioMed Central Ltd.

Abstract

BACKGROUND: Protein acetylation is increasingly recognized as an important mechanism regulating a variety of cellular functions. Several human protein acetyltransferases have been characterized, most of them catalyzing epsilon-acetylation of histones and transcription factors. We recently described the human protein acetyltransferase hARD1 (human Arrest Defective 1). hARD1 interacts with NATH (N-Acetyl Transferase Human) forming a complex expressing protein N-terminal alpha-acetylation activity. RESULTS: We here describe a human protein, hARD2, with 81 % sequence identity to hARD1. The gene encoding hARD2 most likely originates from a eutherian mammal specific retrotransposition event. hARD2 mRNA and protein are expressed in several human cell lines. Immunoprecipitation experiments show that hARD2 protein potentially interacts with NATH, suggesting that hARD2-NATH complexes may be responsible for protein N-alpha-acetylation in human cells. In NB4 cells undergoing retinoic acid mediated differentiation, the level of endogenous hARD1 and NATH protein decreases while the level of hARD2 protein is stable. CONCLUSION: A human protein N-alpha-acetyltransferase is herein described. ARD2 potentially complements the functions of ARD1, adding more flexibility and complexity to protein N-alpha-acetylation in human cells as compared to lower organisms which only have one ARD.

Recommended Citation

Arnesen, Thomas; Betts, Matthew J; Pendino, Frédéric; Liberles, David A; Anderson, Dave; Caro, Jaime; Kong, Xianguo; Varhaug, Jan E; and Lillehaug, Johan R, "Characterization of hARD2, a processed hARD1 gene duplicate, encoding a human protein N-alpha-acetyltransferase." (2006). Department of Medicine Faculty Papers. Paper 43.
https://jdc.jefferson.edu/medfp/43

PubMed ID

16638120