Document Type
Article
Publication Date
3-1-2016
Abstract
Targeted cancer therapeutics are promised to have a major impact on cancer treatment and survival. Successful application of these novel treatments requires a molecular definition of a patient's disease typically achieved through the use of tissue biopsies. Alternatively, allowing longitudinal monitoring, biomarkers derived from blood, isolated either from circulating tumor cell derived DNA (ctcDNA) or circulating cell-free tumor DNA (ccfDNA) may be evaluated. In order to use blood derived templates for mutational profiling in clinical decisions, it is essential to understand the different template qualities and how they compare to biopsy derived template DNA as both blood-based templates are rare and distinct from the gold-standard. Using a next generation re-sequencing strategy, concordance of the mutational spectrum was evaluated in 32 patient-matched ctcDNA and ccfDNA templates with comparison to tissue biopsy derived DNA template. Different CTC antibody capture systems for DNA isolation from patient blood samples were also compared. Significant overlap was observed between ctcDNA, ccfDNA and tissue derived templates. Interestingly, if the results of ctcDNA and ccfDNA template sequencing were combined, productive samples showed similar detection frequency (56% vs 58%), were temporally flexible, and were complementary both to each other and the gold standard. These observations justify the use of a multiple template approach to the liquid biopsy, where germline, ctcDNA, and ccfDNA templates are employed for clinical diagnostic purposes and open a path to comprehensive blood derived biomarker access.
Recommended Citation
Strauss, William M; Carter, Chris; Simmons, Jill; Klem, Erich; Goodman, Nathan; Vahidi, Behrad; Romero, Juan; Masterman-Smith, Michael; O'Regan, Ruth; Gogineni, Keerthi; Schwartzberg, Lee; Austin, Laura; Dempsey, Paul W; and Cristofanilli, Massimo, "Analysis of tumor template from multiple compartments in a blood sample provides complementary access to peripheral tumor biomarkers." (2016). Kimmel Cancer Center Faculty Papers. Paper 62.
https://jdc.jefferson.edu/kimmelccfp/62
Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.
PubMed ID
27049831
Comments
This article has been peer reviewed. It was published in: Oncotarget.
Volume 7, Issue 18, 1 May 2016, Pages 26724-26738.
The published version is available at DOI: 10.18632/oncotarget.8494
Copyright © 2016 The Authors