Document Type
Article
Publication Date
2-18-2022
Abstract
There is growing appreciation for astrocyte heterogeneity both across and within central nervous system (CNS) regions, as well as between intact and diseased states. Recent work identified multiple astrocyte subpopulations in mature brain. Interestingly, one subpopulation (Population C) was shown to possess significantly enhanced synaptogenic properties in vitro, as compared with other astrocyte subpopulations of adult cortex and spinal cord. Following spinal cord injury (SCI), damaged neurons lose synaptic connections with neuronal partners, resulting in persistent functional loss. We determined whether SCI induces an enhanced synaptomodulatory astrocyte phenotype by shifting toward a greater proportion of Population C cells and/or increasing expression of relevant synapse formation-associated genes within one or more astrocyte subpopulations. Using flow cytometry and RNAscope in situ hybridization, we found that astrocyte subpopulation distribution in the spinal cord did not change to a selectively synaptogenic phenotype following mouse cervical hemisection-type SCI. We also found that spinal cord astrocytes expressed synapse formation-associated genes to a similar degree across subpopulations, as well as in an unchanged manner between uninjured and SCI conditions. Finally, we confirmed these astrocyte subpopulations are also present in the human spinal cord in a similar distribution as mouse, suggesting possible conservation of spinal cord astrocyte heterogeneity across species.
Recommended Citation
Allahyari, R Vivian; Heinsinger, Nicolette M; Hwang, Daniel; Jaffe, David A; Rasouli, Javad; Shiers, Stephanie; Thomas, Samantha J; Price, Theodore J; Rostami, Abdolmohamad; and Lepore, Angelo C, "Response of Astrocyte Subpopulations Following Spinal Cord Injury." (2022). Farber Institute for Neuroscience Faculty Papers. Paper 42.
https://jdc.jefferson.edu/farberneursofp/42
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
PubMed ID
35203371
Language
English
Comments
This article is the author's final published version in Cells, Volume 11, Issue 4, February 2022, Article number 721.
The published version is available at https://doi.org/10.3390/cells11040721.
Copyright © 2022 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).