Investigating Clot-Flow Interactions by Integrating Intravital Imaging with in Silico Modeling for Analysis of Flow, Transport, and Hemodynamic Forces

Authors

Chayut Teeraratkul
Maurizio Tomaiuolo, Thomas Jefferson UniversityFollow
Timothy Stalker, Thomas Jefferson UniversityFollow
Debanjan Mukherjee

Document Type

Article

Publication Date

1-6-2024

Comments

This article is the author's final published version in Scientific Reports, Volume 14, Issue 1, 2024, Article number 696.

The published version is available at https://doi.org/10.1038/s41598-023-49945-x.

Copyright © The Author(s) 2024

Abstract

As a blood clot forms, grows, deforms, and embolizes following a vascular injury, local clot-flow interactions lead to a highly dynamic flow environment. The local flow influences transport of biochemical species relevant for clotting, and determines the forces on the clot that in turn lead to clot deformation and embolization. Despite this central role, quantitative characterization of this dynamic clot-flow interaction and flow environment in the clot neighborhood remains a major challenge. Here, we propose an approach that integrates dynamic intravital imaging with computer geometric modeling and computational flow and transport modeling to develop a unified in silico framework to quantify the dynamic clot-flow interactions. We outline the development of the methodology referred to as Intravital Integrated In Silico Modeling or IVISim, and then demonstrate the method on a sample set of simulations comprising clot formation following laser injury in two mouse cremaster arteriole injury model data: one wild-type mouse case, and one diYF knockout mouse case. Simulation predictions are verified against experimental observations of transport of caged fluorescent Albumin (cAlb) in both models. Through these simulations, we illustrate how the IVISim methodology can provide insights into hemostatic processes, the role of flow and clot-flow interactions, and enable further investigations comparing and contrasting different biological model scenarios and parameter variations.

Recommended Citation

Teeraratkul, Chayut; Tomaiuolo, Maurizio; Stalker, Timothy; and Mukherjee, Debanjan, "Investigating Clot-Flow Interactions by Integrating Intravital Imaging with in Silico Modeling for Analysis of Flow, Transport, and Hemodynamic Forces" (2024). Cardeza Foundation for Hematologic Research. Paper 78.
https://jdc.jefferson.edu/cardeza_foundation/78

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 License.

Language

English