Platelet Glycoprotein lib: Chromosomal Localization and Tissue Expression

Authors

Paul F. Bray, University ofCalifornia, San FranciscoFollow
Jean-Philippe Rosa, University of Texas Health Science CenterFollow
Geoffrey I. Johnston, University of Texas Health Science Center
Donny T. Shiu, University ofCalifornia, San Francisco
Richard G. Cook, Baylor College ofMedicine
Chris Lau, University ofCalifornia, San Francisco
Yuet Wai Kan, University ofCalifornia, San FranciscoFollow
Rodger P. McEver, University of Texas Health Science Center at HoustonFollow
Marc A. Shuman, University ofCalifornia, San FranciscoFollow

Document Type

Article

Publication Date

12-1987

Comments

This article has been peer reviewed. It is the author’s final published version in Journal of Clinical Investigation, Volume 80, Issue 6, December 1987, Pages 1812-1817.

The published version is available at DOI: 10.1172/JCI113277. Copyright © American Society for Clinical Investigation

Abstract

The GPIIb-IIIa complex functions as a receptor for cytoadhesive proteins on the platelet surface. Both GPIIb and GPIIIa are synthesized by a human erythroleukemia (HEL) cell line. We isolated several cDNA clones by screening a HEL cell cDNA library with an oligonucleotide derived from amino acid sequence of GPIIb. Nucleotide and amino acid sequences were determined from 703 bp of one of these clones. Amino acid sequence of purified platelet GPIIb peptides confirmed the identity of the clone. The cDNA encodes the carboxyl terminus of the large (a) subunit of GPIIb and all of the smaller (f6) subunit of GPIIb. By hybridizing the cDNA directly to chromosomes separated by dual laser chromosome sorting, the gene for GPIIb was mapped to chromosome 17. Northern blot analysis showed a - 3.4-kb GPIIb mRNA in HEL cells. We also compared the amino acid sequences determined from eight additional platelet GPIIb peptides with the derived amino acids from a published HEL cell GPIIb cDNA, and the platelet and HEL cell proteins appear to be the same. Despite previous reports that vascular endothelial cells and monocytes contain GPIIb, no GPIIb mRNA was observed in either type of cell. Thus, GPIIb appears to be specific for the platelet-megakaryocyte membrane and is distinct from the a subunits of the adhesion receptors in other normal tissues.

Recommended Citation

Bray, Paul F.; Rosa, Jean-Philippe; Johnston, Geoffrey I.; Shiu, Donny T.; Cook, Richard G.; Lau, Chris; Kan, Yuet Wai; McEver, Rodger P.; and Shuman, Marc A., "Platelet Glycoprotein lib: Chromosomal Localization and Tissue Expression" (1987). Cardeza Foundation for Hematologic Research. Paper 15.
https://jdc.jefferson.edu/cardeza_foundation/15