Document Type

Article

Publication Date

9-16-2026

Comments

This article is the author's final published version in Nature Communications, Volume 17, Issue 1, 2026, Article number 10891.

The published version is available at https://doi.org/10.1038/s41467-026-77901-6. Copyright © The Author(s) 2026.

Abstract

Studies of post-replicative chromatin exclusively focus on the kinetics of protein binding after DNA replication to understand maintenance of transcriptional programs. Here we investigated both chromatin-bound factors and the three-dimensional architecture of the post-replicative genome in human cells. We found that transcription factors and chromatin looping proteins rapidly associate with nascent DNA. We discovered that the architectural factors, CTCF and YY1, bind to their target sequences immediately following DNA replication, and that chromatin loops from 39 to 425 kb re-form within 10 min of DNA replication. This suggests that the 3D structure of chromatin is at most briefly disrupted by DNA replication and quickly restores following replisome passage, providing insight into an unaddressed question of the structure of post-replicated chromatin. Our results suggest that all components of the transcriptional network and tertiary chromatin structure are restored shortly after replication, which is consistent with previously detected fast resumption of transcription on daughter DNA strands.

Creative Commons License

Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

PubMed ID

42893985

Language

English

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