Document Type
Article
Publication Date
January 2007
Abstract
The effect of paromomycin on the interaction of ribosomal subunits was studied. Paromomycin inhibited the antiassociation activity of initiation factor 3 (IF3). Furthermore, ribosomal subunits were associated to form 70S ribosomes by paromomycin even in the presence of 1 mM Mg(2+). Paromomycin did not inhibit the binding of IF3 to the 30S ribosomal subunits. On the other hand, IF3 bound to the 30S subunits was expelled by paromomycin-induced subunit association (70S formation). These results indicate that the stabilization of 70S ribosomes by paromomycin may in part be responsible for its inhibitory effects on translocation and ribosome recycling.
Recommended Citation
Hirokawa, Go; Kaji, Hideko; and Kaji, Akira, "Inhibition of antiassociation activity of translation initiation factor 3 by paromomycin" (2007). Department of Biochemistry and Molecular Biology Faculty Papers. Paper 13.
https://jdc.jefferson.edu/bmpfp/13
Comments
This article has been peer-reviewed. It is the final version as published in Antimicrobial Agents and Chemotherapy, 51(1):175-180, January 2007. It is also available from the publisher's website at http://dx.doi.org/10.1128/AAC.01096-06. Copyright © American Society for Microbiology.