Document Type
Article
Publication Date
9-30-2022
Abstract
Canonical RNA processing in mammalian mitochondria is defined by tRNAs acting as recognition sites for nucleases to release flanking transcripts. The relevant factors, their structures, and mechanism are well described, but not all mitochondrial transcripts are punctuated by tRNAs, and their mode of processing has remained unsolved. Using Drosophila and mouse models, we demonstrate that non-canonical processing results in the formation of 3' phosphates, and that phosphatase activity by the carbon catabolite repressor 4 domain-containing family member ANGEL2 is required for their hydrolysis. Furthermore, our data suggest that members of the FAST kinase domain-containing protein family are responsible for these 3' phosphates. Our results therefore propose a mechanism for non-canonical RNA processing in metazoan mitochondria, by identifying the role of ANGEL2.
Recommended Citation
Clemente, Paula; Calvo-Garrido, Javier; Pearce, Sarah F.; Schober, Florian A.; Shigematsu, Megumi; Siira, Stefan J.; Laine, Isabelle; Spåhr, Henrik; Steinmetzger, Christian; Petzold, Katja; Kirino, Yohei; Wibom, Rolf; Rackham, Oliver; Filipovska, Aleksandra; Rorbach, Joanna; Freyer, Christoph; and Wredenberg, Anna, "ANGEL2 Phosphatase Activity is Required for Non-Canonical Mitochondrial RNA Processing" (2022). Computational Medicine Center Faculty Papers. Paper 42.
https://jdc.jefferson.edu/tjucompmedctrfp/42
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
PubMed ID
36180430
Language
English
Comments
This is the author's final published version available in Nature Communications, Volume 13, Issue 1, Dec. 2022, Article Number 5750.
The published version is available online at https://doi.org/10.1038/s41467-022-33368-9. Copyright © The Author(s) 2022.