Document Type
Article
Publication Date
1-24-2025
Abstract
BACKGROUND: Ataxia telangiectasia mutated (ATM) kinase plays a critical role in DNA double-strand break (DSB) repair. Ataxia telangiectasia (A-T) patients exhibit abnormalities in immunoglobulin isotype expression and class switch recombination (CSR). This study investigates the role of residual ATM kinase expression and activity in the severity of A-T disease.
METHODS: A-T patients with defined genetic diagnoses were classified based on CSR and based on the severity of their medical complications. Isolated peripheral blood mononuclear cells from any patient were evaluated before and after exposure to 0.5 Gy ionizing radiation for one minute. Western blotting was performed to identify the expression of ATM and phosphorylated ATM (p-ATM) proteins compared to age-sex-matched healthy controls.
RESULTS: In severe A-T patients (n = 6), the majority (66.7%) had frameshift mutations, while 33.3% had nonsense mutations in the ATM gene. The mild group (n = 3) had two cases of splice errors and one missense mutation. All patients with CSR defect had elevated IgM serum levels, whereas all switched immunoglobulins were reduced in them. Expression of ATM and p-ATM proteins was significantly lower (p = 0.01) in all patients compared to healthy controls, both pre-and post- and post-radiation. Additionally, low ATM and p-ATM protein expression levels were linked with the clinical severity of patients but were not correlated with CSR defects.
CONCLUSION: Expression and activation of ATM protein were defective in A-T patients compared to healthy controls. Altered expression of ATM and p-ATM proteins may have potential clinical implications for prognostic evaluation and symptom severity assessment in individuals with A-T.
Recommended Citation
Salami, Fereshte; Shad, Tannaz Moeini; Fathi, Nazanin; Mojtahedi, Hanieh; Esmaeili, Marzie; Shahkarami, Sepideh; Afrakoti, Ladan Gol Mohammad Pour; Amirifar, Parisa; Delavari, Samaneh; Nosrati, Hassan; Razavi, Azadehsadat; Ranjouri, Mohammad Reza; Yousefpour, Mahsa; Esfahani, Zahra Hamidi; Azizi, Gholamreza; Ashrafi, Mahmoudreza; Rezaei, Nima; Yazdani, Reza; and Abolhassani, Hassan, "ATM Expression and Activation in Ataxia Telangiectasia Patients with and without Class Switch Recombination Defects" (2025). Department of Neurology Faculty Papers. Paper 380.
https://jdc.jefferson.edu/neurologyfp/380
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
PubMed ID
39853455
Language
English
Included in
Biological Phenomena, Cell Phenomena, and Immunity Commons, Diseases Commons, Genetic Phenomena Commons, Neurology Commons
Comments
This article is the author’s final published version in Journal of Clinical Immunology, Volume 45, Issue 1, 2025, Article number 67.
The published version is available at https://doi.org/10.1007/s10875-025-01857-3. Copyright © The Author(s) 2025.