Document Type
Report
Publication Date
4-25-2023
Abstract
Hepatitis B virus (HBV)-host junction sequences (HBV-JSs) has been detected in the urine of patients with HBV infection. This study evaluated HBV-JSs as a marker of minimum residual disease (MRD) and tumor recurrence after treatment in HBV-hepatocellular carcinoma (HCC) patients. Archived serial urine DNA from two HBV–HCC with recurrence as confirmed by MRI and four HBV-related cirrhosis (LC) patients were used. Urinary HBV-JSs were identified by an HBV-targeted NGS assay. Quantitative junction-specific PCR assays were developed to investigate dynamic changes of the most abundant urinary HBV-JS. Abundant urinary HBV-JSs were identified in two cases of tumor recurrence. In case 1, a 78-year-old female with HBV- HCC underwent a follow-up MRI following microwave ablation. While MRI results were variable, the unique HBV-JS DNA, HBV-Chr17, steadily increased from initial diagnosis to HCC recurrence. In case 2, a 74-year-old male with HBV–HCC contained two HBV-JS DNA, HBV-Chr11 and HBV-TERT, that steadily increased after initial HCC diagnosis till recurrence. One LC examined had HBV-TERT DNA detected, but transiently in 3.5 years during HCC surveillance. HBV-JS DNA was persistently elevated prior to the diagnosis of recurrent HCC, suggesting the potential of urinary HBV-JS DNA to detect MRD and HCC recurrence after treatment.
Recommended Citation
Lin, Selena Y; Halegoua-De Marzio, Dina; Block, Peter; Kao, Yu-Lan; Civan, Jesse M.; Shieh, Fwu-Shan; Song, Wei; Hann, Hie-Won; and Su, Ying-Hsiu, "Persistently Elevated HBV Viral-Host Junction DNA in Urine as a Biomarker for Hepatocellular Carcinoma Minimum Residual Disease and Recurrence: A Pilot Study" (2023). Department of Medicine Faculty Papers. Paper 411.
https://jdc.jefferson.edu/medfp/411
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
PubMed ID
37174929
Language
English
Comments
This article is the author's final published version in Diagnostics, Volume 13, Issue 9, 2023, Article number 1537.
The published version is available at https://doi.org/10.3390/diagnostics13091537.
Copyright © 2023 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).